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使用[具体物质]绿色合成的氧化锌纳米颗粒对非洲绿猴肾细胞的细胞毒性。 （你提供的原文中“using”后面似乎缺少具体内容）

Cytotoxicity of green synthesized zinc oxide nanoparticles using on Vero cells.

作者信息

Maheswaran Harshyini, Djearamane Sinouvassane, Tanislaus Antony Dhanapal Anto Cordelia, Wong Ling Shing

机构信息

Department of Biomedical Sciences, Faculty of Science, Universiti Tunku Abdul Rahman (UTAR), Kampar Campus, Jalan Universiti, Bandar Barat, 31900, Kampar, Perak, Malaysia.

Biomedical Research Unit and Lab Animal Research Centre, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, 602 105, India.

出版信息

Heliyon. 2024 May 15;10(11):e31316. doi: 10.1016/j.heliyon.2024.e31316. eCollection 2024 Jun 15.

DOI:10.1016/j.heliyon.2024.e31316
PMID:38868065
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11167271/
Abstract

Zinc oxide nanoparticles (ZnO NPs) have become a highly regarded substance in various industries especially biologically synthesized ZnO NPs due to their adherence to the principles of green chemistry. However, concerns have been raised regarding the potential cytotoxic effects of ZnO NPs on biological systems. This study aimed to investigate and compare the cytotoxicity of ZnO NPs that were synthesized through chemical (C-ZnO NPs) and green approach using leaf aqueous extract (Ma-ZnO NPs) on Vero cells. Characterization of ZnO NPs through Uv-Vis, FESEM, EDX, XRD, FTIR and XPS confirmed the successful synthesis of C- and Ma-ZnO NPs. MTT and ROS assays revealed that C- and Ma-ZnO NPs induced a concentration- and time-dependent cytotoxic effect on Vero cells. Remarkably, Ma-ZnO NPs showed significantly higher cell viability compared to C-ZnO NPs. The corelation of ROS and vell viability suggest that elevated ROS levels can lead to cell damage and even cell death. Flow cytometry analysis indicated that Ma-ZnO NPs exposed cells had more viable cells and a smaller cell population in the late and early apoptotic stage. Furthermore, more cells were arrested in the G1 phase upon exposure to C-ZnO NPs, which is associated with oxidative stress and DNA damage caused by ROS generation, proving its higher cytotoxicity than Ma-ZnO NPs. Similarly, time-dependent cytotoxicity and morphological alterations were observed in C- and Ma-ZnO NPs treated cells, indicating cellular damage. Furthermore, fluorescence microscopy also demonstrated a time-dependent increase in ROS formation in cells exposed to C- and Ma-ZnO NPs. In conclusion, the findings suggest that green ZnO NPs possess a favourable biocompatibility profile, exhibiting reduced cytotoxicity compared to chemically synthesized ZnO NPs on Vero cells. These results emphasize the potential of green synthesis methods for the development of safer and environmentally friendly ZnO NPs.

摘要

氧化锌纳米颗粒（ZnO NPs）已成为各个行业备受关注的物质，尤其是生物合成的ZnO NPs，因为它们符合绿色化学原则。然而，人们对ZnO NPs对生物系统的潜在细胞毒性作用表示担忧。本研究旨在研究和比较通过化学方法（C-ZnO NPs）和使用树叶水提取物的绿色方法（Ma-ZnO NPs）合成的ZnO NPs对Vero细胞的细胞毒性。通过紫外可见光谱、场发射扫描电子显微镜、能谱分析、X射线衍射、傅里叶变换红外光谱和X射线光电子能谱对ZnO NPs进行表征，证实了C-和Ma-ZnO NPs的成功合成。MTT和活性氧（ROS）检测表明，C-和Ma-ZnO NPs对Vero细胞具有浓度和时间依赖性的细胞毒性作用。值得注意的是，与C-ZnO NPs相比，Ma-ZnO NPs显示出显著更高的细胞活力。ROS与细胞活力的相关性表明，ROS水平升高会导致细胞损伤甚至细胞死亡。流式细胞术分析表明，暴露于Ma-ZnO NPs的细胞具有更多的活细胞，且处于晚期和早期凋亡阶段的细胞群体较小。此外，暴露于C-ZnO NPs时，更多细胞停滞在G1期，这与ROS产生引起的氧化应激和DNA损伤有关，证明其细胞毒性高于Ma-ZnO NPs。同样，在C-和Ma-ZnO NPs处理的细胞中观察到时间依赖性的细胞毒性和形态改变，表明细胞受到损伤。此外，荧光显微镜也显示，暴露于C-和Ma-ZnO NPs的细胞中ROS形成呈时间依赖性增加。总之，研究结果表明，绿色ZnO NPs具有良好的生物相容性，与化学合成的ZnO NPs相比，对Vero细胞的细胞毒性降低。这些结果强调了绿色合成方法在开发更安全、环境友好型ZnO NPs方面的潜力。

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