Shaanxi University of Science & Technology, Xi'an 710021, China.
Shaanxi University of Science & Technology, Xi'an 710021, China.
Int J Biol Macromol. 2019 Nov 1;140:196-205. doi: 10.1016/j.ijbiomac.2019.08.139. Epub 2019 Aug 17.
A porous microsphere with good biocompatibility was fabricated based on collagen (COL) and bacterial cellulose (BC). The adsorption and release behaviors of the COL/BC porous microspheres were studied using BSA as the model protein, and employing quasi-primary, quasi-secondary, and Kannan-Sundaram intragranular diffusion models, zero-order, first-order, Higuchi and Korsmeyer-Peppas models. The results showed that the COL/BC porous microspheres are beneficial to the proliferation of MC3T3 E1-cells. The linear Langmuir equation can accurately describe the adsorption equilibrium relationship of BSA to the COL/BC microspheres. The pseudo-second-order model can more accurately explain and predict the membrane diffusion kinetics of BSA than both pseudo-primary-order and Kannan-Sundaram intragranular diffusion models. The adsorption rate was affected by both membrane and intragranular diffusions. The drug release behavior indicated that the microsphere-loaded BSA was primarily adsorbed at the inner wall of the pore, and exhibited the characteristics of a scaffold-based matrix meanwhile. The drug release kinetics can be accurately described by the first-order release model. The present study elucidated the mechanism of drug adsorption and release of COL/BC porous microspheres and provided a theoretical basis for its application in controlled release technology.
基于胶原蛋白（COL）和细菌纤维素（BC）制备了一种具有良好生物相容性的多孔微球。采用 BSA 作为模型蛋白，研究了 COL/BC 多孔微球的吸附和释放行为，并采用准一级、准二级、Kannan-Sundaram 颗粒内扩散模型、零级、一级、Higuchi 和 Korsmeyer-Peppas 模型进行了研究。结果表明，COL/BC 多孔微球有利于 MC3T3 E1 细胞的增殖。线性 Langmuir 方程可以准确描述 BSA 对 COL/BC 微球的吸附平衡关系。与准一级和 Kannan-Sundaram 颗粒内扩散模型相比，伪二级模型能更准确地解释和预测 BSA 的膜扩散动力学。吸附速率受膜扩散和颗粒内扩散的共同影响。药物释放行为表明，载有 BSA 的微球主要吸附在孔的内壁上，同时表现出支架基质的特征。药物释放动力学可以通过一级释放模型准确描述。本研究阐明了 COL/BC 多孔微球的药物吸附和释放机制，为其在控制释放技术中的应用提供了理论依据。
