Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing, China.
Key Laboratory of Modern Precision Agriculture System Integration Research, Ministry of Education, China Agricultural University, Beijing, China.
J Virol Methods. 2019 Nov;273:113723. doi: 10.1016/j.jviromet.2019.113723. Epub 2019 Aug 17.
Rapid screening of infectious viral diseases is the key to ensure healthy development of duck livestock industry. Currently routine viral detection methods are primarily used to detect up to 3 viruses. In this study, matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) was used for simultaneous detection and genotyping of ten viruses in duck, including Duck hepatitis A virus 1 (DHAV-1), DHAV-3, Duck astrovirus 1 (DAstV-1), DAstV-2, Duck reovirus 1 (DRV-1), DRV-2, Tembusu virus (TMUV), Avian influenza virus (AIV), Goose parvovirus (GPV) and Duck enteritis virus (DEV). The low detection limits of this proposed method for ten duck viruses ranged from 1.3 copies/μl to 7.8 copies/μl. The novel detection method with high sensitivity, good specificity and high throughput has the potential to be applied for disease diagnosis and surveillance.
快速筛查传染性病毒疾病是确保鸭养殖业健康发展的关键。目前，常规病毒检测方法主要用于检测多达 3 种病毒。本研究采用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）技术，同时检测和基因分型鸭的 10 种病毒，包括鸭甲型肝炎病毒 1 型（DHAV-1）、DHAV-3、鸭星状病毒 1 型（DAstV-1）、DAstV-2、鸭呼肠孤病毒 1 型（DRV-1）、DRV-2、坦布苏病毒（TMUV）、禽流感病毒（AIV）、鹅细小病毒（GPV）和鸭肠炎病毒（DEV）。该方法对 10 种鸭病毒的最低检测限为 1.3 拷贝/μl 至 7.8 拷贝/μl。该新型检测方法具有高灵敏度、良好特异性和高通量的特点，有望应用于疾病诊断和监测。
