School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
Joint International Research Laboratory of Agriculture and Agri-Product Safety (The Ministry of Education), Yangzhou University, Yangzhou 225009, China.
Int J Mol Sci. 2022 Jan 7;23(2):648. doi: 10.3390/ijms23020648.
Respiratory burst oxidase homologs (Rbohs) are critical enzymes involved in the generation of reactive oxygen species (ROS) that play an important role in plant growth and development as well as various biotic and abiotic stresses in plants. Thus far, there have been few reports on the characterization of the gene family in . In this study, seven genes (~) were identified in the genome. The CsRboh proteins were predicted to localize to the cell membrane. Most CsRbohs contained four conserved domains, an EF-hand domain, and a transmembrane region. Phylogenetic analysis demonstrated that the CsRbohs were divided into five groups, suggesting potential distinct functions and evolution. The expression profiles revealed that these seven genes displayed tissue-specific expression patterns, and five genes were responsive to cold stress. Fourteen putative -acting elements related to stress response, hormone response, and development regulation were present within the promoters of genes. The in-silico microRNA target transcript analyses indicated that might be targeted by csi-miR164. Further functional and physiological analyses showed that the knockdown of in trifoliate orange impaired resistance to cold stress. As a whole, our results provide valuable information for further functional studies of the genes in response to cold stress.
呼吸爆发氧化酶同源物（Rbohs）是参与活性氧（ROS）生成的关键酶，在植物生长发育以及各种生物和非生物胁迫中起着重要作用。迄今为止，关于 基因家族的特征描述报告较少。本研究在 基因组中鉴定出了 7 个 基因（~）。CsRboh 蛋白被预测定位于细胞膜。大多数 CsRbohs 包含四个保守结构域，一个 EF 手结构域和一个跨膜区域。系统发育分析表明，CsRbohs 分为五个组，表明潜在的不同功能和进化。表达谱显示这七个 基因表现出组织特异性表达模式，其中五个 基因对冷胁迫有响应。在 基因启动子中存在 14 个与应激响应、激素响应和发育调控相关的假定 - 作用元件。计算机模拟的 microRNA 靶转录分析表明 可能是 csi-miR164 的靶标。进一步的功能和生理分析表明，三裂橘中 的敲低会损害其对冷胁迫的抗性。总的来说，我们的结果为进一步研究 基因对冷胁迫的功能提供了有价值的信息。
