Lee Gyeong Seok, Yang Hyeon Gung, Kim Ji Hun, Ahn Young Mo, Han Man Deuk, Kim Wan Jong
Department of Life Science and Biotechnology , Soonchunhyang University , Asan , Korea . Conception and design; acquisition, analysis and interpretation of data; technical procedures; histopathological examinations; statistics analysis; manuscript writing, final approval.
Department of Life Science and Biotechnology , Soonchunhyang University , Asan , Korea . Conception and design; acquisition, analysis and interpretation of data; technical procedures; histopathological examinations; statistics analysis; manuscript preparation and writing, final approval.
Acta Cir Bras. 2019 Aug 19;34(6):e201900606. doi: 10.1590/s0102-865020190060000006.
To investigate the effects of pine needle extract (PNE) on the expression of proliferating cell nuclear antigen (PCNA) and Ki-67 during liver regeneration induced by 70% partial hepatectomy (PH) in rat.
Forty-eight male rats (SD, 7 weeks) had surgery (70% PH). They were randomly divided into two groups. PH + PNE group was only provided PNE diluted in water (10%) for drinking and PH group was provided water from 5 days before surgery to the time of sacrifice. PNE was made by pressing and filtering. Animals were sacrificed at 12h, 24h, 36h, 60h, 84h, 168h after PH, respectively. The expressions of PCNA and Ki-67 were determined as proliferation indices.
Immunohistochemistry turned out to increase the expression of PCNA and Ki-67. PCNA expression of PH+PNE group increased up to twice of that of PH group. Western blot also seemed to increase the PCNA expression. These results indicated the promotion of cell proliferation in liver tissue and hepatic regeneration.
Pine needle extract stimulates the expression of some mitotic proteins during liver regeneration induced by 70% PH in rats. It suggests that administration of pine needle extract could accelerate the liver regeneration after partial hepatectomy.
研究松针提取物（PNE）对大鼠70%部分肝切除（PH）诱导的肝再生过程中增殖细胞核抗原（PCNA）和Ki-67表达的影响。
48只雄性大鼠（7周龄，SD大鼠）接受手术（70% PH）。将它们随机分为两组。PH + PNE组仅提供用10%水稀释的PNE饮用，PH组从手术前5天至处死时均提供水。PNE通过压榨和过滤制成。分别在PH后12小时、24小时、36小时、60小时、84小时、168小时处死动物。将PCNA和Ki-67的表达作为增殖指标进行测定。
免疫组织化学显示PCNA和Ki-67的表达增加。PH + PNE组的PCNA表达增加至PH组的两倍。蛋白质印迹法似乎也增加了PCNA的表达。这些结果表明肝组织中细胞增殖和肝再生得到促进。
松针提取物在大鼠70% PH诱导的肝再生过程中刺激某些有丝分裂蛋白的表达。这表明给予松针提取物可以加速部分肝切除术后的肝再生。
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