Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Molecular & Cell Biology Research Center, Student Research Committee, School of Medicine, Mazandaran University of Medical Science, Sari, Iran.
Anticancer Agents Med Chem. 2020;20(8):963-969. doi: 10.2174/1871520620666200311095836.
Nowadays the use of plant-derived products has been extensively examined in the treatment of many types of gastrointestinal cancers such as hepatocarcinoma and colon cancer. Urtica dioica is a traditional herb that has many pharmacological effects and wildly used as a therapeutic agent in cancer. Herein, we have evaluated the effects of the different concentrations of Methanolic Extract of Urtica dioica (MEUD) on viability, death pattern, and expression of the apoptosis-related gene in normal Human Dermal Fibroblast (HDF), hepatocarcinoma cell lines (HepG2) and colon-cancer cell line (HCT116).
A high-performance liquid chromatography method was developed to simultaneously separate 3 phenolic acids in MEUD. HepG2 and HCT116 cell lines as well as HDF normal cell line were cultured in suitable media. After 24 and 48h, in the cultured cell with different concentrations of MEUD, cells viability was assessed by MTT assay, and apoptosis was also evaluated at the cellular level by Annexin V/PI flow cytometry analyzing and AO/EB staining. BCL2 and BAX gene expressions were assessed by TaqMan real-time PCR assay.
MEUD showed antiproliferative effects on HepG2 and HTC116 cells after 48h with an IC50 value of about 410 and 420μg/ml, respectively (P < 0.001). Apoptotic cells were observed in HepG2 and HTC116 cells but not in HDF. Furthermore, the increased level of BAX/BCL-2 ratio was observed in HepG2 and HTC116 cells under the treatment of different concentrations of MEUD.
The MEUD may influence hepatocarcinoma and colon-cancer cell lines at specific doses and change their proliferation rate by changing the expression of BAX and BCL2.
如今，植物衍生产品在治疗多种类型的胃肠道癌，如肝癌和结肠癌方面的应用得到了广泛的研究。荨麻是一种具有多种药理作用的传统草药，被广泛用作癌症的治疗剂。在此，我们评估了不同浓度的荨麻甲醇提取物（MEUD）对正常人类真皮成纤维细胞（HDF）、肝癌细胞系（HepG2）和结肠癌细胞系（HCT116）活力、死亡模式和凋亡相关基因表达的影响。
建立了一种同时分离 MEUD 中 3 种酚酸的高效液相色谱法。将 HepG2 和 HCT116 细胞系以及 HDF 正常细胞系在合适的培养基中培养。用不同浓度的 MEUD 培养细胞 24 和 48 小时后，通过 MTT 法评估细胞活力，并通过 Annexin V/PI 流式细胞术分析和 AO/EB 染色在细胞水平评估细胞凋亡。通过 TaqMan 实时 PCR 法评估 BCL2 和 BAX 基因的表达。
MEUD 对 HepG2 和 HTC116 细胞在 48 小时后表现出增殖抑制作用，其 IC50 值分别约为 410 和 420μg/ml（P<0.001）。在 HepG2 和 HTC116 细胞中观察到凋亡细胞，但在 HDF 中未观察到。此外，在不同浓度的 MEUD 处理下，HepG2 和 HTC116 细胞中 BAX/BCL-2 比值升高。
MEUD 可能在特定剂量下影响肝癌和结肠癌细胞系，并通过改变 BAX 和 BCL2 的表达来改变其增殖率。
