使用API 20 Strep系统鉴定杰氏棒状杆菌和棒状杆菌CDC D2群。

Identification of Corynebacterium jeikeium and Corynebacterium CDC group D2 with the API 20 Strep system.

作者信息

Tillotson G, Arora M, Robbins M, Holton J

机构信息

API/Bio Merieux Ltd., Basingstoke, Hampshire, UK.

出版信息

Eur J Clin Microbiol Infect Dis. 1988 Oct;7(5):675-8. doi: 10.1007/BF01964252.

DOI:10.1007/BF01964252

PMID:3143582

Abstract

A total of 170 strains of Corynebacterium jeikeium and 23 strains of Corynebacterium group D2 were examined in three British laboratories using the API 20 Strep identification system and three supplementary tests (catalase production, urease production and nitrate reduction). The isolates were collected from clinical specimens in various laboratories over a three-year period. The two species produced consistent reactions in these tests after 24 h. Two tests were highly discriminatory, with positive reactions for ribose fermentation seen for Corynebacterium jeikeium while urease production was observed with Corynebacterium group D2. This method allows routine clinical laboratories to rapidly identify these emerging pathogens.

摘要

英国的三个实验室使用API 20 Strep鉴定系统和三项补充试验（过氧化氢酶产生、脲酶产生和硝酸盐还原），对总共170株杰氏棒状杆菌和23株D2群棒状杆菌进行了检测。这些分离株是在三年时间里从各个实验室的临床标本中收集的。这两个菌种在24小时后的这些试验中产生了一致的反应。两项试验具有高度鉴别性，杰氏棒状杆菌对核糖发酵呈阳性反应，而D2群棒状杆菌则观察到脲酶产生。这种方法使常规临床实验室能够快速鉴定这些新出现的病原体。