Soto A, Zapardiel J, Soriano F
Department of Clinical Microbiology, Fundación Jiménez Díaz, Madrid, Spain.
J Clin Pathol. 1994 Aug;47(8):756-9. doi: 10.1136/jcp.47.8.756.
To identify rapidly and accurately coryneform bacteria, using a commercial strip system.
Ninety eight strains of Corynebacterium species and 62 additional strains belonging to genera Erysipelothrix, Oerskovia, Rhodococcus, Actinomyces, Archanobacterium, Gardnerella and Listeria were studied. Bacteria were identified using conventional biochemical tests and a commercial system (API-Coryne, BioMèrieux, France). Fresh rabbit serum was added to fermentation tubes for Gardnerella vaginalis isolates.
One hundred and five out of the 160 (65.7%) organisms studied were correctly and completely identified by the API Coryne system. Thirty five (21.8%) more were correctly identified with additional tests. Seventeen (10.6%) organisms were not identified by the system and three (1.9%) were misidentified.
The system was a good alternative for identification of coryneform organisms. When occasionally performed with some additional tests, this method permits reliable and rapid identification of coryneform organisms compared with conventional methods.
使用一种商业纸条系统快速准确地鉴定棒状杆菌。
研究了98株棒状杆菌属菌株以及另外62株属于丹毒丝菌属、厄氏菌属、红球菌属、放线菌属、隐秘杆菌属、加德纳菌属和李斯特菌属的菌株。使用传统生化试验和一种商业系统(API - Coryne,法国生物梅里埃公司)对细菌进行鉴定。对于阴道加德纳菌分离株,在发酵管中加入新鲜兔血清。
所研究的160株菌中,有105株(65.7%)通过API Coryne系统得到正确和完整的鉴定。通过额外试验又正确鉴定出35株(21.8%)。该系统未能鉴定出17株(10.6%)菌,误鉴定了3株(1.9%)。
该系统是鉴定棒状杆菌的一个良好替代方法。与传统方法相比,偶尔结合一些额外试验使用这种方法能够可靠且快速地鉴定棒状杆菌。