黑腹果蝇中葡萄糖脱氢酶基因的分子结构与转化
Molecular structure and transformation of the glucose dehydrogenase gene in Drosophila melanogaster.
作者信息
Whetten R, Organ E, Krasney P, Cox-Foster D, Cavener D
机构信息
Department of Molecular Biology, Vanderbilt University, Nashville, Tennessee 37235.
出版信息
Genetics. 1988 Oct;120(2):475-84. doi: 10.1093/genetics/120.2.475.
Abstract
We have precisely mapped and sequenced the three 5' exons of the Drosophila melanogaster Gld gene and have identified the start sites for transcription and translation. The first exon is composed of 335 nucleotides and does not contain any putative translation start codons. The second exon is separated from the first exon by 8 kb and contains the Gld translation start codon. The inferred amino acid sequence of the amino terminus contains two unusual features: three tandem repeats of serine-alanine, and a relatively high density of cysteine residues. P element-mediated transformation experiments demonstrated that a 17.5-kb genomic fragment contains the functional and regulatory components of the Gld gene.
摘要
我们已经精确绘制了黑腹果蝇Gld基因的三个5'外显子图谱并进行了测序，还确定了转录和翻译的起始位点。第一个外显子由335个核苷酸组成，不包含任何假定的翻译起始密码子。第二个外显子与第一个外显子相隔8 kb，包含Gld翻译起始密码子。氨基末端的推断氨基酸序列包含两个不同寻常的特征：丝氨酸 - 丙氨酸的三个串联重复序列，以及相对高密度的半胱氨酸残基。P因子介导的转化实验表明，一个17.5 kb的基因组片段包含Gld基因的功能和调控元件。
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