Rheumatology Unit, Department of Medicine, University of Leipzig, Leipzig, Germany.
Division of Endocrinology, Department of Medicine, University of Leipzig, Leipzig, Germany.
Front Immunol. 2019 Aug 8;10:1874. doi: 10.3389/fimmu.2019.01874. eCollection 2019.
Circulating monocytes can be divided into classical (CM), intermediate (IM), and non-classical monocytes (NCM), and the classical monocytes also contain CD56+ monocytes and monocytic myeloid-derived suppressor cells (M-MDSC). The aim of the study was to evaluate the occurrence of the monocyte subpopulations in human obesity. Twenty-seven normal, 23 overweight, and 60 obese individuals (including 17 obese individuals with normal glucose tolerance and 27 with type 2 diabetes) were included into this study. Peripheral blood mononuclear cells were isolated from human blood, and surface markers to identify monocyte subpopulations were analyzed by flow cytometry. Obese individuals had higher numbers of total monocytes, CM, IM, CD56+ monocytes, and M-MDSCs. The number of CM, IM, CD56+ monocytes, and M-MDSCs, correlated positively with body mass index, body fat, waist circumference, triglycerides, C-reactive protein, and HbA1c, and negatively with high-density lipoprotein cholesterol. Individuals with obesity and type 2 diabetes had higher numbers of IM, NCM, and M-MDSCs, whereas those with obesity and impaired glucose tolerance had higher numbers of CD56+ monocytes. In summary, the comprehensive analysis of blood monocytes in human obesity revealed a shift of the monocyte compartment toward pro-inflammatory monocytes which might contribute to the development of low-grade inflammation in obesity, and immune-suppressive monocytes which might contribute to the development of cancer in obesity.
循环中的单核细胞可分为经典型（CM）、中间型（IM）和非经典型单核细胞（NCM），经典型单核细胞还包含 CD56+单核细胞和单核细胞源性髓系抑制细胞（M-MDSC）。本研究旨在评估人肥胖症中单核细胞亚群的发生情况。本研究纳入了 27 名正常体重者、23 名超重者和 60 名肥胖者（包括 17 名糖耐量正常的肥胖者和 27 名 2 型糖尿病肥胖者）。从人血中分离外周血单核细胞，并用流式细胞术分析鉴定单核细胞亚群的表面标志物。肥胖个体的总单核细胞、CM、IM、CD56+单核细胞和 M-MDSC 数量较高。CM、IM、CD56+单核细胞和 M-MDSC 的数量与体重指数、体脂肪、腰围、甘油三酯、C 反应蛋白和 HbA1c 呈正相关，与高密度脂蛋白胆固醇呈负相关。肥胖伴 2 型糖尿病个体的 IM、NCM 和 M-MDSC 数量较高，而肥胖伴糖耐量受损个体的 CD56+单核细胞数量较高。总之，对人肥胖症血液单核细胞的综合分析显示，单核细胞群向促炎型单核细胞发生偏移，这可能有助于肥胖症中低度炎症的发生，而免疫抑制型单核细胞可能有助于肥胖症中癌症的发生。
