Medical College, Shandong University, Jinan, China.
Department of General Surgery, Anhui Provincial Hospital, No. 17 Lujiang Road, Hefei, 230001, China.
Dig Dis Sci. 2020 Feb;65(2):489-499. doi: 10.1007/s10620-019-05797-y. Epub 2019 Aug 22.
The Suvar, Enhancer of zeste, and Trithorax (SET), myeloid-Nervy-DEAF-1 (MYND) domain-containing protein 3 (SMYD3), was reported to be upregulated in various tumors. However, its role in pancreatic cancer progression remains unclear.
To explore the role of SMYD3 in the pancreatic cancer.
The expressions of SMYD3, caspase-3, and matrix metallopeptidase-2 (MMP-2) were detected in pancreatic cancer and non-tumor tissues by immunohistochemistry. The CCK-8 and transwell assays were performed to test proliferation, migration, and invasion ability in short hairpin RNA (shRNA-SMYD3) pancreatic cancer cell line SW1190. RT-PCR and Western blot were used to detect the expressions of SMYD3, caspase-3, and MMP-2 in SW1990 cell line and shRNA-SMYD3 SW1990 cell line.
The expressions of SMYD3, caspase-3, and MMP-2 were upregulated in pancreatic cancer. The SMYD3 was positively associated with caspase-3 and MMP-2 expressions in pancreatic cancer tissues. SMYD3, TNM stages, histological differentiation, and lymph node metastasis were identified as an independent prognostic factor. Moreover, interfered SMYD3 expression in SW1990 cell line significantly reduced the cell proliferation, migration, and invasion. RT-PCR and Western blot showed the expression of MMP-2 decreased in shRNA-SMYD3 SW1990 cell line, but no significant change was observed in the caspase-3 expression.
The overexpression of SMYD3 promoted proliferation, migration, and invasion of pancreatic cancer, and SMYD3 may affect the pancreatic cancer progression by regulating MMP-2 rather than caspase-3.
Suvar、 Enhancer of zeste、Trithorax（SET）和髓样神经耳聋 1（MYND）结构域蛋白 3（SMYD3）在各种肿瘤中均被报道上调。然而，其在胰腺癌进展中的作用尚不清楚。
探讨 SMYD3 在胰腺癌中的作用。
采用免疫组织化学法检测胰腺癌和非肿瘤组织中 SMYD3、半胱氨酸天冬氨酸蛋白酶-3（caspase-3）和基质金属蛋白酶-2（MMP-2）的表达。采用 CCK-8 法和 Transwell 小室实验检测短发夹 RNA（shRNA-SMYD3）胰腺癌 SW1190 细胞系的增殖、迁移和侵袭能力。采用 RT-PCR 和 Western blot 检测 SW1990 细胞系和 shRNA-SMYD3 SW1990 细胞系中 SMYD3、caspase-3 和 MMP-2 的表达。
SMYD3、caspase-3 和 MMP-2 在胰腺癌中表达上调。SMYD3 在胰腺癌组织中与 caspase-3 和 MMP-2 的表达呈正相关。SMYD3、TNM 分期、组织学分化和淋巴结转移被确定为独立的预后因素。此外，干扰 SW1990 细胞系中 SMYD3 的表达显著降低了细胞增殖、迁移和侵袭。RT-PCR 和 Western blot 显示，shRNA-SMYD3 SW1990 细胞系中 MMP-2 的表达降低，但 caspase-3 的表达无明显变化。
SMYD3 的过表达促进了胰腺癌的增殖、迁移和侵袭，SMYD3 可能通过调节 MMP-2 而不是 caspase-3 影响胰腺癌的进展。
