College of Life Science, Shanxi University, Taiyuan 030000, China.
Shanxi Key Laboratory for Research and Development of Regional Plants, Taiyuan 030000, China.
Molecules. 2020 Apr 12;25(8):1767. doi: 10.3390/molecules25081767.
Ultrasound-assisted extraction (UAE) was applied to extract rutin (RU), nicotiflorin (NI), narcissoside (NA), kaempferol (KA), isorhamnetin (IS), quercetin (QU), and total flavonoids of Flos Sophorae Immaturus (TFFSI) from Flos Sophorae Immaturus (FSI). Through single factor test and response surface methodology (RSM), the optimal extraction conditions were concluded as follows: ethanol concentration 70%, time 30 min, temperature 61 °C, and liquid/solid ratio 15.30 mL/g, respectively. The actual extraction rates of RU, NI, NA, KA, IS, QU, and TFFSI were 14.6101%, 2.9310%, 7.1987%, 0.1041%, 0.4920%, 2.7998%, and 26.4260%, respectively. The experimental results demonstrated that the extraction method with accuracy and efficiency could be used for the comprehensive evaluation quality control of extracts from FSI. The antioxidant activities of hydroalcoholic extraction from FSI on 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•), superoxide anion (•O) free radicals, and ferric reducing/antioxidant power (FRAP) were assessed. The results showed that the antioxidation activities of extracts on DPPH, ABTS•, and •O free radicals were reached 89.29%, 97.86%, and 56.61%, and 81.4% in FRAP at 1.0 mg/mL, respectively. The antioxidant capacity of FSI extract was positively correlated with the amount of total flavonoids.
超声辅助提取（UAE）被应用于从槐米中提取芦丁（RU）、槲皮苷（NI）、水仙苷（NA）、山奈酚（KA）、异鼠李素（IS）、槲皮素（QU）和槐米总黄酮（TFFSI）。通过单因素试验和响应面法（RSM），得出了最佳提取条件如下：乙醇浓度 70%，时间 30 分钟，温度 61°C，液固比 15.30 mL/g。RU、NI、NA、KA、IS、QU 和 TFFSI 的实际提取率分别为 14.6101%、2.9310%、7.1987%、0.1041%、0.4920%、2.7998%和 26.4260%。实验结果表明，该提取方法准确、高效，可用于槐米提取物的综合评价和质量控制。槐米水醇提取物对 1,1-二苯基-2-苦基肼基（DPPH）、2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS•）、超氧阴离子（•O）自由基和铁还原/抗氧化能力（FRAP）的抗氧化活性进行了评估。结果表明，提取物对 DPPH、ABTS•和•O 自由基的抗氧化活性分别达到 89.29%、97.86%和 56.61%，在 1.0mg/mL 时 FRAP 达到 81.4%。槐米提取物的抗氧化能力与总黄酮含量呈正相关。
