REQUIMTE/LAQV, Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, R. Jorge Viterbo Ferreira, n° 228, 4050-313 Porto, Portugal.
Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100 Murcia, Spain.
Biomolecules. 2019 Aug 22;9(9):400. doi: 10.3390/biom9090400.
The economic value of fig trees has been globally acknowledged due to their utilization in the food industry, being also frequently used in traditional medicine. While ubiquitously distributed in Southeast Asia, Corner remains uninvestigated concerning its biological properties and chemical profile. HPLC-DAD-ESI/MS characterization of methanol extracts obtained from the stem bark and leaves allowed the identification and quantitation of 21 phenolic compounds for the first time; the stem bark was predominantly rich in flavan-3-ols and apigenin derivatives, while solely apigenin-di-glycosides have been identified and quantitated on the leaf extract. Both extracts inhibited 5-lipoxygenase (5-LOX) activity in a concentration-dependent manner, the one obtained from the stem bark being significantly more active (IC = 10.75 μg/mL). The effect of both extracts on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages was evaluated, and while the stem bark extract did not lead to a noticeable interference on nitric oxide (NO) levels, the extract obtained from the leaves notably decreased NO and L-citrulline levels at concentrations ranging from 250 to 500 μg/mL. Herein, is valorized due to its modulatory effects on inflammatory mediators and also as a source of bioactive phenols, which may fuel further studies on the development of nutraceuticals.
由于其在食品工业中的应用，以及在传统医学中的频繁使用，无患子树的经济价值在全球范围内得到了认可。虽然无患子树在东南亚广泛分布，但Corner 地区尚未对其生物特性和化学特征进行研究。通过高效液相色谱-二极管阵列检测-电喷雾/质谱联用（HPLC-DAD-ESI/MS）对其茎皮和叶甲醇提取物进行分析，首次鉴定并定量了 21 种酚类化合物；茎皮主要富含黄烷-3-醇和芹菜素衍生物，而叶提取物中仅鉴定和定量了芹菜素二糖苷。两种提取物均以浓度依赖的方式抑制 5-脂氧合酶（5-LOX）活性，其中茎皮提取物的活性显著更强（IC = 10.75 μg/mL）。还评估了两种提取物对脂多糖（LPS）刺激的 RAW 264.7 巨噬细胞的影响，虽然茎皮提取物对一氧化氮（NO）水平没有明显的干扰作用，但叶提取物在 250 至 500 μg/mL 的浓度范围内显著降低了 NO 和 L-瓜氨酸的水平。因此，Corner 地区的无患子树因其对炎症介质的调节作用以及作为生物活性酚类物质的来源而具有价值，这可能为开发营养保健品的进一步研究提供了依据。
