Woodford N, Ison C A
Department of Medical Microbiology, Wright-Fleming Institute, St. Mary's Hospital Medical School, London, UK.
J Antimicrob Chemother. 1988 Oct;22(4):463-71. doi: 10.1093/jac/22.4.463.
We have investigated the effect of four different media on the results of susceptibility tests for clinical and reference strains of Neisseria gonorrhoeae. Diagnostic Sensitivity Test (DST) agar supplemented with 5% lysed horse blood and 1% IsoVitaleX was regarded as the 'standard' and three other media were compared with it--DST plus 5% lysed blood, GC agar supplemented with 1% IsoVitaleX and Proteose agar plus 1% haemoglobin and 1% IsoVitaleX. We found no significant differences between the two DST media when testing penicillin, cefuroxime, tetracycline or erythromycin. Proteose agar compared well with the 'standard' for all antibiotics except erythromycin for which MICs were significantly increased. GC agar showed the poorest agreement with the 'standard' medium. Gonococci appeared more sensitive to tetracycline and less sensitive to erythromycin on this medium. The use of GC agar did not significantly affect susceptibilities to the beta-lactam agents tested, although MICs measured on GC agar did tend to be higher than on the 'standard' DST medium.
我们研究了四种不同培养基对淋病奈瑟菌临床菌株和参考菌株药敏试验结果的影响。添加5%裂解马血和1%异维生素K的诊断敏感性试验（DST）琼脂被视为“标准”培养基，并将其他三种培养基与之进行比较，即添加5%裂解血的DST培养基、添加1%异维生素K的GC琼脂以及添加1%血红蛋白和1%异维生素K的蛋白胨琼脂。在检测青霉素、头孢呋辛、四环素或红霉素时，我们发现两种DST培养基之间没有显著差异。除红霉素外，蛋白胨琼脂与“标准”培养基对所有抗生素的检测结果都比较相似，而在蛋白胨琼脂上红霉素的最低抑菌浓度（MIC）显著升高。GC琼脂与“标准”培养基的一致性最差。在此培养基上，淋球菌对四环素似乎更敏感，而对红霉素不太敏感。使用GC琼脂对所检测的β-内酰胺类药物的敏感性没有显著影响，尽管在GC琼脂上测得的MIC往往高于“标准”DST培养基。
