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源自羊膜（HAMSCs）和脐带（HUC-MSCs）的人骨髓间充质干细胞的表型特征和成骨分化潜能

Phenotype Characteristics and Osteogenic Differentiation Potential of Human Mesenchymal Stem Cells Derived from Amnion Membrane (HAMSCs) and Umbilical Cord (HUC-MSCs).

作者信息

Hendrijantini Nike, Hartono Poedjo

机构信息

Department of Prosthodontic, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.

Department of Obstetrics and Gynaecology, Dr. Soetomo General Hospital, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.

出版信息

Acta Inform Med. 2019 Jun;27(2):72-77. doi: 10.5455/aim.2019.27.72-77.

DOI:10.5455/aim.2019.27.72-77
PMID:31452562
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6688306/
Abstract

INTRODUCTION: Human amnion membrane mesenchymal stem cells (hAMSCs) and human umbilical cord mesenchymal stem cells (hUC-MSCs) are potential, non invasive sources of stem cells used for bone tissue engineering. Phenotyping characterization is an extremely important consideration in the choice of the appropriate passage in order to maximize its osteogenic differentiation potential. AIM: To explore phenotype characteristics and compare osteogenic differentiation potential of hAMSCs and hUC-MSCs. METHOD: Isolation and culture were performed on hAMSCs and hUC-MSCs from a healthy woman in her 38 weeks of pregnancy. CD90, CD105 and CD73 phenotype characterization was done in passage 4-7. An osteogenic differentiation examination of hAMSCs and hUC-MSCs with Alizarin red staining and RUNX2 expression was performed in the passage that had appropriate expressions of phenotype characteristics. RESULTS: The expression of CD90 hUC-MSCs was higher than that of hAMSCs in all passages. CD105 hUC-MSCs was higher in passage 4-6, while CD105 hAMSCs was equal to that of hUC-MSCs in passage 7. CD73 hUC-MSCs was higher than hAMSCs in passage 4 and 5, while in passage 6 and 7 hAMSCs was higher than hUC-MSCs. There was a decrease in the number of CD90, CD105 and CD73 on hAMSCs and hUC-MSCs in passage 5, then determined as appropriate passage. Alizarin red staining examination showed calcium deposition and revealed no significant difference, but RUNX2 expression of hUC-MSCs was significantly higher than that for hAMSCs. CONCLUSION: Both hAMSCs and hUC-MSCs had phenotype characteristics of mesenchymal stem cell and showed ostegenic differentiation potential.

摘要

引言：人羊膜间充质干细胞（hAMSCs）和人脐带间充质干细胞（hUC-MSCs）是用于骨组织工程的潜在、非侵入性干细胞来源。在选择合适的传代次数时，表型特征是一个极其重要的考虑因素，以便最大限度地发挥其成骨分化潜能。 目的：探讨hAMSCs和hUC-MSCs的表型特征并比较其成骨分化潜能。 方法：从一名怀孕38周的健康女性中分离并培养hAMSCs和hUC-MSCs。在第4 - 7代进行CD90、CD105和CD73表型特征鉴定。在具有合适表型特征表达的传代中，对hAMSCs和hUC-MSCs进行茜素红染色和成骨分化相关基因RUNX2表达的检测。 结果：在所有传代中，hUC-MSCs的CD90表达均高于hAMSCs。hUC-MSCs的CD105在第4 - 6代较高，而hAMSCs的CD105在第7代与hUC-MSCs相等。hUC-MSCs的CD73在第4代和第5代高于hAMSCs，而在第6代和第7代hAMSCs高于hUC-MSCs。在第5代时，hAMSCs和hUC-MSCs上的CD90、CD105和CD73数量减少，随后确定为合适的传代。茜素红染色检测显示钙沉积且无显著差异，但hUC-MSCs的RUNX2表达明显高于hAMSCs。 结论：hAMSCs和hUC-MSCs均具有间充质干细胞的表型特征，并显示出成骨分化潜能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de1b/6688306/68ddd63fe2e4/AIM-27-72-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de1b/6688306/44f011d7042f/AIM-27-72-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de1b/6688306/2011bfef2f1b/AIM-27-72-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de1b/6688306/b48a0fae14e2/AIM-27-72-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de1b/6688306/ed3872e12710/AIM-27-72-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de1b/6688306/68ddd63fe2e4/AIM-27-72-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de1b/6688306/44f011d7042f/AIM-27-72-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de1b/6688306/2011bfef2f1b/AIM-27-72-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de1b/6688306/b48a0fae14e2/AIM-27-72-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de1b/6688306/ed3872e12710/AIM-27-72-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de1b/6688306/68ddd63fe2e4/AIM-27-72-g005.jpg

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