用于无标记、低葡萄糖浓度检测的光流体微毛细管生物传感器。
Optofluidic microcapillary biosensor for label-free, low glucose concentration detection.
作者信息
Wan Hongdan, Chen Jijing, Wan Cheng, Zhou Quan, Wang Jie, Zhang Zuxing
机构信息
Nanjing University of Posts and Telecommunications, Nanjing 210023, China.
Department of Biomedical Engineering, College of Engineering of Peking University, Beijing, China.
出版信息
Biomed Opt Express. 2019 Jul 12;10(8):3929-3937. doi: 10.1364/BOE.10.003929. eCollection 2019 Aug 1.
Abstract
We demonstrate label-free detection of low glucose concentration based on whispery gallery mode resonance in an optofluidic microcapillary (OFMC) biosensor. The wall surface of the OFMC is bio-chemically functionalized to detect cellular-level glucose concentration as low as 2.78 mM. The measured sensitivity is 0.966 pm/mM. The fabricated microcapillary has a thin wall thickness of 2 μm and a Q factor of 1.3 × 10, corresponding to a bulk refractive index sensitivity of 23.36 nm/RIU. The OFMC biosensor has advantages such as high resistance to environmental perturbation, small sample volume down to 90 nL and cost-effectiveness. Theoretical analysis shows the sensor sensitivity depends on the microcapillary wall thickness and liquid core refractive index.
摘要
我们展示了基于光流体微毛细管（OFMC）生物传感器中低语回廊模式共振的低葡萄糖浓度无标记检测。OFMC的壁表面经过生物化学功能化处理，以检测低至2.78 mM的细胞水平葡萄糖浓度。测量的灵敏度为0.966 pm/mM。制造的微毛细管壁厚为2μm，品质因数为1.3×10，对应的体折射率灵敏度为23.36 nm/RIU。OFMC生物传感器具有抗环境干扰能力强、样品体积小至90 nL以及成本效益高等优点。理论分析表明，传感器灵敏度取决于微毛细管壁厚和液芯折射率。
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