Zheng Ting, Feng Zhao, Wang Xiaojun, Jiang Tao, Jin Rui, Zhao Peilin, Luo Ting, Gong Hui, Luo Qingming, Yuan Jing
Collaborative Innovation Center for Biomedical Engineering, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.
Britton Chance Center and MOE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.
Biomed Opt Express. 2019 Jul 17;10(8):4075-4096. doi: 10.1364/BOE.10.004075. eCollection 2019 Aug 1.
Elucidating connectivity and functionality at the whole-brain level is one of the most challenging research goals in neuroscience. Various whole-brain optical imaging technologies with submicron lateral resolution have been developed to reveal the fine structures of brain-wide neural and vascular networks at the mesoscopic level. Among them, micro-optical sectioning tomography (MOST) is attracting increasing attention, as a variety of technological variations and solutions tailored toward different biological applications have been optimized. Here, we summarize the recent development of MOST technology in whole-brain imaging and anticipate future improvements.
阐明全脑水平的连通性和功能是神经科学中最具挑战性的研究目标之一。为了在介观水平上揭示全脑范围的神经和血管网络的精细结构，已经开发了各种具有亚微米横向分辨率的全脑光学成像技术。其中，微光学切片断层扫描（MOST）正受到越来越多的关注，因为针对不同生物学应用的各种技术变体和解决方案已经得到优化。在这里，我们总结了MOST技术在全脑成像方面的最新进展，并展望了未来的改进方向。
