孟加拉国迈门辛地区家禽、家蝇和池塘水中耐黏菌素细菌的出现。
Emergence of colistin-resistant in poultry, house flies, and pond water in Mymensingh, Bangladesh.
作者信息
Sobur Md Abdus, Ievy Samina, Haque Zobayda Farzana, Nahar Ashrafun, Zaman Sumaiya Binte, Rahman Md Tanvir
机构信息
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
出版信息
J Adv Vet Anim Res. 2019 Jan 22;6(1):50-53. doi: 10.5455/javar.2019.f311. eCollection 2019 Mar.
OBJECTIVE
Emergence of colistin-resistant (CREC) has generated a sense of public alarm. The objective of this study was to detect the CREC and identification of the gene responsible for such resistance.
MATERIALS AND METHODS
A total of 150 samples comprising poultry cloacal swab, house flies (), and pond water were collected randomly from Mymensingh, Bangladesh and analyzed. Isolation and identification of were done based on culture and 16S rRNA gene-specific polymerase chain reaction (PCR). Phenotypic detection of CREC was done by disk diffusion method. Finally, colistin resistance genes were detected by PCR by using colistin resistant gene specific primers.
RESULTS
Among the 150 samples, phenotypically 18.00% ( = 27/150) isolates were found as colistin resistant. By PCR, 8.00% of the isolates were found positive for the presence of gene.
CONCLUSIONS
Colistin resistant carrying are detected in poultry, house flies and water that are of great public health concern.
目的
耐黏菌素大肠杆菌（CREC）的出现引起了公众的恐慌。本研究的目的是检测CREC并鉴定负责这种耐药性的基因。
材料与方法
从孟加拉国迈门辛随机收集了总共150份样本，包括家禽泄殖腔拭子、家蝇（）和池塘水，并进行了分析。基于培养和16S rRNA基因特异性聚合酶链反应（PCR）对大肠杆菌进行分离和鉴定。通过纸片扩散法对CREC进行表型检测。最后，使用耐黏菌素基因特异性引物通过PCR检测耐黏菌素基因。
结果
在150份样本中，表型上发现18.00%（=27/150）的分离株对黏菌素耐药。通过PCR，8.00%的大肠杆菌分离株被发现携带该基因呈阳性。
结论
在家禽、家蝇和水中检测到携带该基因的耐黏菌素大肠杆菌，这对公众健康具有重大影响。
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