Bahroudi Saeed, Shabanpour Bahareh, Combie Joan, Shabani Ali, Salimi Mona
Department of Fisheries, Gorgan Agricultural Sciences and Natural Resources, Gorgan, Iran.
Montana Biopolymers Inc., 119 Cathcart Circle, Winnsboro, SC 29180, USA.
Iran Biomed J. 2020 Jan;24(1):54-9. doi: 10.29252/ibj.24.1.54. Epub 2019 Aug 28.
Recently, exploring novel dietary nondigestible carbohydrates, which are able to influence the gut flora, has drawn much attention. The objective of this study was to find out the effective dose of levan, as a prebiotic, in rats in order to further apply in food industry.
Levan at various doses (2-10%) was orally administered to male Wistar Albino rats once a day for 90 days. At the end of experiment, fecal and blood samples were collected to measure gut bacteria population and to carry out serum biochemical assay. The rats were sacrificed, and the colon tissues were stained with hematoxylin and eosin and analyzed by histopathology.
Of note, levan effectively controlled body weight gain in the rats. Serum biochemical analysis revealed that 5% levan significantly diminished the serum level of total cholesterol, LDL, and glucose as well. More notably, 5% levan intake significantly increased the abundance of bifidobacteria population, highlighting its bifidogenic effect. Furthermore, our histopathological result revealed that daily intake of levan was associated with a higher degree of thickness of the mucosa layer compared to the rats in control group. Moreover, these findings manifested no colon inflammation in the rats fed with levan.
The findings of this study provide the fundamental data to use levan at a definite dose for further development in functional foods.
最近，探索能够影响肠道菌群的新型膳食不可消化碳水化合物受到了广泛关注。本研究的目的是找出作为益生元的果聚糖在大鼠体内的有效剂量，以便进一步应用于食品工业。
将不同剂量（2%-10%）的果聚糖每天一次口服给予雄性Wistar白化大鼠，持续90天。实验结束时，采集粪便和血液样本以测量肠道细菌数量并进行血清生化分析。处死大鼠，将结肠组织用苏木精和伊红染色并进行组织病理学分析。
值得注意的是，果聚糖有效地控制了大鼠的体重增加。血清生化分析显示，5%的果聚糖显著降低了总胆固醇、低密度脂蛋白和血糖的血清水平。更值得注意的是，摄入5%的果聚糖显著增加了双歧杆菌的数量，突出了其双歧杆菌生成作用。此外，我们的组织病理学结果显示，与对照组大鼠相比，每天摄入果聚糖与更高程度的黏膜层厚度相关。而且，这些发现表明喂食果聚糖的大鼠没有结肠炎症。
本研究结果为使用特定剂量的果聚糖进行功能性食品的进一步开发提供了基础数据。
