Sun Lei, Fu Yuhua, Yang Yang, Wang Xinxin, Cui Weijie, Li Dan, Yuan Xiaohui, Zhang Zhiwu, Fu Yongping, Li Yu
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, China.
School of Computer Science and Technology, Wuhan University of Technology, Wuhan, China.
Front Microbiol. 2019 Aug 13;10:1786. doi: 10.3389/fmicb.2019.01786. eCollection 2019.
distributed in the Tibetan Plateau of China has high-stress resistance that is valuable for breeding improvements. However, its evolutionary history, specialization, and adaptation to the extreme Tibetan Plateau environment are largely unknown. Here, we performed sequencing of a representative Tibetan Plateau wild strain ABM and comparative genomic analysis with the reported European strain H97 and H39. The assembled ABM genome was 30.4 Mb in size, and comprised 8,562 protein-coding genes. The ABM genome shared highly conserved syntenic blocks and a few inversions with H97 and H39. The phylogenetic tree constructed by 1,276 single-copy orthologous genes in nine fungal species showed that the Tibetan Plateau and European diverged ∼5.5 million years ago. Population genomic analysis using genome resequencing of 29 strains revealed that the Tibetan Plateau population underwent significant differentiation from the European and American populations and evolved independently, and the global climate changes critically shaped the demographic history of the Tibetan Plateau population. Moreover, we identified key genes that are related to the cell wall and membrane system, and the development and defense systems regulated adapting to the harsh Tibetan Plateau environment. These findings highlight the value of genomic data in assessing the evolution and adaptation of mushrooms and will enhance future genetic improvements of .
分布于中国青藏高原的[具体物种名称未提及]具有高抗逆性，这对育种改良具有重要价值。然而，其进化历史、特化以及对青藏高原极端环境的适应性在很大程度上尚不清楚。在此，我们对青藏高原代表性野生菌株ABM进行了测序，并与已报道的欧洲菌株H97和H39进行了比较基因组分析。组装后的ABM基因组大小为30.4 Mb，包含8562个蛋白质编码基因。ABM基因组与H97和H39共享高度保守的共线性区域和一些倒位。由9种真菌中的1276个单拷贝直系同源基因构建的系统发育树表明，青藏高原菌株和欧洲菌株在约550万年前分化。利用29个菌株的基因组重测序进行的群体基因组分析表明，青藏高原群体与欧美群体发生了显著分化并独立进化，全球气候变化对青藏高原群体的种群历史产生了关键影响。此外，我们鉴定出了与细胞壁和膜系统以及适应青藏高原恶劣环境的发育和防御系统相关的关键基因。这些发现凸显了基因组数据在评估蘑菇进化和适应性方面的价值，并将促进未来[具体物种名称未提及]的遗传改良。
