Wu Jianhui, Yu Xintong, Wang Xiaoshuang, Tang Liangde, Ali Shaukat
Key Laboratory of Bio-Pesticide Innovation and Application, Engineering Research Centre of Biological Control, South China Agricultural University, Guangzhou, China.
Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture, Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
Front Microbiol. 2019 Aug 13;10:1812. doi: 10.3389/fmicb.2019.01812. eCollection 2019.
The repetitive application of pesticides at high doses against Fabricius has resulted in development of pesticide resistance and harmful effects to the natural environmental. Hence, finding alternate pest control strategies, such as entomopathogenic fungi or their application in combination with other natural chemicals, is of great importance to solve the abovementioned problems. This study presents the toxic effects of and matrine (individual or in combination with each other) against tobacco cutworm (). Different matrine treatments caused a dose dependent increase in mortality at different time intervals. The biological parameters of (germination rate and average daily mycelia growth) were not inhibited by different matrine treatments. Different conidial concentrations of caused significantly different mortalities of 2nd instar larvae at different time intervals. Different combined treatments of and matrine showed a significant synergistic effect against under laboratory and semi-field conditions. The current findings showed a strong synergistic action for combined application of and matrine against Our results will provide baseline information on combined application of entomopathogenic fungi and natural chemicals in integrated pest management programs against .
对棉铃虫大量重复施用高剂量农药已导致其产生抗药性，并对自然环境造成有害影响。因此，寻找替代害虫控制策略，如昆虫病原真菌或其与其他天然化学物质联合应用，对于解决上述问题至关重要。本研究展示了白僵菌和苦参碱（单独或相互组合）对烟草夜蛾的毒性作用。不同苦参碱处理在不同时间间隔导致棉铃虫死亡率呈剂量依赖性增加。白僵菌的生物学参数（发芽率和平均每日菌丝体生长）未受不同苦参碱处理的抑制。不同分生孢子浓度的白僵菌在不同时间间隔对二龄烟草夜蛾幼虫造成的死亡率显著不同。在实验室和半田间条件下，白僵菌与苦参碱的不同组合处理对烟草夜蛾显示出显著的协同效应。当前研究结果表明白僵菌与苦参碱联合应用对烟草夜蛾具有强大的协同作用。我们的结果将为昆虫病原真菌与天然化学物质联合应用于烟草夜蛾综合害虫管理计划提供基础信息。
