Liang Xu, Lee Hui Wen, Li Zhifeng, Lu Yonghai, Zou Li, Ong Choon Nam
NUS Environment Research Institute, National University of Singapore, 5A Engineering Drive 1, 117411 Singapore.
College of Pharmacy, Jiangxi University of Traditional Chinese Medicine, 1688 Meilingdadao Road, 330004 Nanchang, Jiangxi Province, P. R. China.
ACS Omega. 2018 Nov 15;3(11):15546-15553. doi: 10.1021/acsomega.8b01668. eCollection 2018 Nov 30.
Glucosinolates, which are unique to vegetables, have diverse biological activities, including antimicrobial, antioxidant, and anticancer actions. In this study, we applied hydrophilic interaction chromatography-tandem mass spectrometry (HILIC-MS/MS) to the simultaneous quantification of 22 glucosinolates in 12 vegetables, including pak choi, choy sum, Chinese cabbage, cauliflower, cabbage, broccoli, Kai Lan, Brussels sprouts, rocket salad, daikon radish, red cherry radish, and watercress. Significant differences in concentration and composition of glucosinolates were observed among these vegetables. Cabbage had the highest level of total glucosinolates (μg/g dry weight: 19 551.2 ± 1317.7), whereas Kai Lan had the lowest level (7611.3 ± 868.4). Aliphatic and indole glucosinolates were the major components in the 12 vegetables ranging from 76 to 100%, except watercress (37%). On the basis of the content of glucosinolates, the 12 vegetables were well distinguishable and classified according to their morphological taxonomy. This study presents a HILIC-MS/MS approach for quantification of glucosinolates, and demonstrates the potential of glucosinolate profiles for species identification.
硫代葡萄糖苷是蔬菜特有的成分，具有多种生物活性，包括抗菌、抗氧化和抗癌作用。在本研究中，我们应用亲水相互作用色谱-串联质谱法（HILIC-MS/MS）同时定量分析了12种蔬菜中的22种硫代葡萄糖苷，这些蔬菜包括小白菜、菜心、大白菜、花椰菜、甘蓝、西兰花、芥蓝、抱子甘蓝、芝麻菜、白萝卜、红樱桃萝卜和西洋菜。这些蔬菜中硫代葡萄糖苷的浓度和组成存在显著差异。甘蓝的总硫代葡萄糖苷含量最高（微克/克干重：19551.2±1317.7），而芥蓝的含量最低（7611.3±868.4）。除西洋菜（37%）外，脂肪族和吲哚类硫代葡萄糖苷是这12种蔬菜中的主要成分，占比76%至100%。基于硫代葡萄糖苷的含量，这12种蔬菜根据其形态分类法能够很好地区分和归类。本研究提出了一种用于硫代葡萄糖苷定量分析的HILIC-MS/MS方法，并证明了硫代葡萄糖苷谱在物种鉴定方面的潜力。
