Mousseau Fanny, Berret Jean-François, Oikonomou Evdokia K
Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS Université Denis Diderot Paris-VII, Bâtiment Condorcet, 10 rue Alice Domon et Léonie Duquet, 75205 Paris, France.
ACS Omega. 2019 Jun 17;4(6):10485-10493. doi: 10.1021/acsomega.9b01094. eCollection 2019 Jun 30.
Amphiphilic molecules such as surfactants, lipids, and block copolymers can be assembled into bilayers and form vesicles. Fluorescent membrane labeling methods require the use of dye molecules that can be inserted into the bilayers at different stages of synthesis. To our knowledge, there is no generalized method for labeling preformed vesicles. Herein, we develop a versatile protocol that is suitable to both surfactant and lipid preformed vesicles and requires no separation or purification steps. On the basis of the lipophilic carbocyanine green dye PKH67, the methodology is assessed on zwitterionic phosphatidylcholine vesicles. To demonstrate its versatility, it is applied to dispersions of anionic or cationic vesicles, such as a drug administrated to premature infants with respiratory distress syndrome, or a vesicle formulation used as a fabric softener for home care applications. By means of fluorescence microscopy, we then visualize the interaction mechanisms of nanoparticles crossing live cell membranes and of surfactants adsorbed on a cotton fabric. These results highlight the advantages of a membrane labeling technique that is simple and applicable to a large number of soft matter systems.
两亲性分子，如表面活性剂、脂质和嵌段共聚物，可以组装成双层膜并形成囊泡。荧光膜标记方法需要使用能够在合成的不同阶段插入双层膜的染料分子。据我们所知，目前还没有用于标记预制囊泡的通用方法。在此，我们开发了一种通用方案，该方案适用于表面活性剂和脂质预制囊泡，且无需分离或纯化步骤。基于亲脂性碳菁绿染料PKH67，该方法在两性离子磷脂酰胆碱囊泡上进行了评估。为了证明其通用性，将其应用于阴离子或阳离子囊泡的分散体，如用于患有呼吸窘迫综合征的早产儿的药物，或用作家庭护理应用中的织物柔软剂的囊泡制剂。然后，通过荧光显微镜，我们可视化了纳米颗粒穿过活细胞膜以及表面活性剂吸附在棉织物上的相互作用机制。这些结果突出了一种简单且适用于大量软物质系统的膜标记技术的优点。
