CD206⁺CD14⁺巨噬细胞在肺癌所致恶性胸腔积液诊断中的价值
Diagnostic value of CD206CD14 macrophages in diagnosis of lung cancer originated malignant pleural effusion.
作者信息
Pei Xue-Bin, Wu Xiu-Zhi, Yi Feng-Shuang, Zhai Kan, Shi Huan-Zhong
机构信息
Department of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China.
出版信息
J Thorac Dis. 2019 Jul;11(7):2730-2736. doi: 10.21037/jtd.2019.06.44.
BACKGROUND
Pleural effusions are common complications of various diseases. Patients with malignant pleural effusion (MPE) usually face poor prognosis and short life expectancy. Discriminating between MPE and benign pleural effusion remains to be difficult. The aim of our current study was to evaluate whether CD206CD14 macrophages could be a diagnostic biomarker for MPE.
METHODS
The percentages of CD14, CD86CD14 and CD206CD14 macrophages in pleural effusions were determined by flow cytometry in 34 patients with MPE and 26 with benign pleural effusion, and their diagnostic performances were evaluated by receiver operating characteristic (ROC) curves.
RESULTS
The percentages of CD14, CD86CD14 and CD206CD14 macrophages were remarkably higher in MPE than in benign pleural effusion (all P<0.05). With a cutoff value of 39.8%, a high sensitivity of 88.2% and high specificity of 100.0% were found in CD206CD14 macrophages to diagnose MPE. The area under the curve, positive predictive value and negative predictive value of CD206CD14 macrophages were 0.980 (95% CI, 0.905 to 0.999), 100.0 (88.4 to 100.0) and 86.7 (69.3 to 96.2), respectively. The diagnostic performance of CD206CD14 macrophages was more accurate than those of CD14 and CD86CD14 macrophages.
CONCLUSIONS
CD206CD14 macrophages could be used to discriminate MPE from benign pleural effusion.
背景
胸腔积液是多种疾病的常见并发症。恶性胸腔积液（MPE）患者通常预后较差，预期寿命较短。鉴别MPE和良性胸腔积液仍然困难。我们当前研究的目的是评估CD206⁺CD14⁺巨噬细胞是否可作为MPE的诊断生物标志物。
方法
采用流式细胞术测定34例MPE患者和26例良性胸腔积液患者胸腔积液中CD14⁺、CD86⁺CD14⁺和CD206⁺CD14⁺巨噬细胞的百分比，并通过受试者工作特征（ROC）曲线评估其诊断性能。
结果
MPE中CD14⁺、CD86⁺CD14⁺和CD206⁺CD14⁺巨噬细胞的百分比显著高于良性胸腔积液（均P<0.05）。以39.8%为临界值，发现CD206⁺CD14⁺巨噬细胞诊断MPE的灵敏度为88.2%，特异性为100.0%。CD206⁺CD14⁺巨噬细胞的曲线下面积、阳性预测值和阴性预测值分别为0.980（95%CI，0.905至0.999）、100.0（88.4至100.0）和86.7（69.3至96.2）。CD206⁺CD14⁺巨噬细胞的诊断性能比CD14⁺和CD86⁺CD14⁺巨噬细胞更准确。
结论
CD206⁺CD14⁺巨噬细胞可用于鉴别MPE和良性胸腔积液。
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