Laboratory of Bio-Physio Sensors and Nano-bioengineering, Department of Bioscience and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, 781039, India.
Mikrochim Acta. 2019 Aug 29;186(9):658. doi: 10.1007/s00604-019-3773-2.
A nonenzymatic electrochemical nanoprobe is described for the fast determination of hydrogen peroxide (HO). A sputtered indium tin oxide electrode with a nano-hierarchical 3D gold structure is used. The nanoprobe was characterized by SEM, EDX, TEM, SAED, and electrochemical techniques. Figures of merit include (a) a fast response time (≤ 1.0 s), (b) two linear dynamic ranges that extend from 10 M to 10 M and from 10 M to10 M; and (c) a low limit of detection of 9.8 × 10 M. The nanoprobe works in the clinical range and was applied for trace analysis of HO in spiked blood samples, and recoveries ranged between 90 and 96%. It has negligible response (p < 0.001, for n = 3) toward glucose, citric acid, ascorbic acid, uric acid, glycine, and alanine. The shelf-lifetime is found to be 12 weeks. Graphical abstract Schematic representation of a dendritic nanochip with peroxidase-like activity. It is made from an indium tin oxide electrode with a nanohierarchical gold structure and was used for amperometric determination of hydrogen peroxide.
一种非酶电化学纳米探针，用于快速测定过氧化氢（HO）。使用了溅射氧化铟锡电极和纳米分级 3D 金结构。通过 SEM、EDX、TEM、SAED 和电化学技术对纳米探针进行了表征。优点包括：(a) 快速响应时间（≤1.0 s）；(b) 两个线性动态范围，从 10-6 M 扩展到 10-5 M 和从 10-5 M 扩展到 10-4 M；(c) 检测限低至 9.8×10-6 M。该纳米探针在临床范围内工作，并应用于加标血液样本中 HO 的痕量分析，回收率在 90%至 96%之间。它对葡萄糖、柠檬酸、抗坏血酸、尿酸、甘氨酸和丙氨酸的响应可以忽略不计（p<0.001，n=3）。货架寿命发现为 12 周。
