Mogale Motetelo Alfred, Mhlanga Catherine Martha, Gololo Stanley Sechene, Adu Agustine
Department of Biochemistry, School of Science and Technology, Sefako Makgatho Health Sciences University, Pretoria, South Africa. Email:
Department of Biochemistry, School of Science and Technology, Sefako Makgatho Health Sciences University, Pretoria, South Africa.
Cardiovasc J Afr. 2019;30(4):193-197. doi: 10.5830/CVJA-2018-060. Epub 2019 Aug 2.
This case-control study investigated the association between major types of serum advanced glycation end-products (AGEs) and selected serum/plasma markers of endothelial dysfunction in black patients with type 2 diabetes mellitus at Dr George Mukhari Academic Hospital. Serum AGEs were measured using either enzyme-linked immunosorbent assay (ELISA) or spectrofluoremetry. Serum markers of endothelial dysfunction were measured using either ELISA or calometry. The correlation and associations between major types of serum AGEs and markers of endothelial dysfunction were investigated using the Spearman correlation coefficient and bivariate logistic regression analysis, respectively. Although both serum total immunogenic AGEs and serum carboxymethyl-lysine (CML) were moderately and negatively associated with endothelial dysfunction, only serum CML was significantly associated with a higher odds for the development of endothelial dysfunction (low nitric oxide levels) in our diabetic subjects. It can therefore be concluded from this study that high serum levels of CML may predispose to endothelial dysfunction in black South Africans with type 2 diabetes.
这项病例对照研究在乔治·穆卡里学术医院对患有2型糖尿病的黑人患者进行了调查，以探究血清晚期糖基化终产物（AGEs）的主要类型与选定的内皮功能障碍血清/血浆标志物之间的关联。血清AGEs采用酶联免疫吸附测定法（ELISA）或荧光分光光度法进行测量。内皮功能障碍的血清标志物采用ELISA或比色法进行测量。分别使用Spearman相关系数和二元逻辑回归分析来研究血清AGEs主要类型与内皮功能障碍标志物之间的相关性和关联性。尽管血清总免疫原性AGEs和血清羧甲基赖氨酸（CML）均与内皮功能障碍呈中度负相关，但在我们的糖尿病受试者中，只有血清CML与内皮功能障碍（低一氧化氮水平）发生的较高几率显著相关。因此，从这项研究可以得出结论，高血清CML水平可能使南非黑人2型糖尿病患者易患内皮功能障碍。
