Ram Hasthi, Soni Praveen, Salvi Prafull, Gandass Nishu, Sharma Ankita, Kaur Amandeep, Sharma Tilak Raj
Department of Agri-Biotechnology, National Agri-Food Biotechnology Institute (NABI), Sector-81, SAS Nagar, Mohali 140306, India.
Department of Botany, Rajasthan University, Jaipur 302004, India.
Plants (Basel). 2019 Aug 29;8(9):310. doi: 10.3390/plants8090310.
Insertional mutagenesis is an indispensable tool for engendering a mutant population using exogenous DNA as the mutagen. The advancement in the next-generation sequencing platform has allowed for faster screening and analysis of generated mutated populations. Rice is a major staple crop for more than half of the world's population; however, the functions of most of the genes in its genome are yet to be analyzed. Various mutant populations represent extremely valuable resources in order to achieve this goal. Here, we have reviewed different insertional mutagenesis approaches that have been used in rice, and have discussed their principles, strengths, and limitations. Comparisons between transfer DNA (T-DNA), transposons, and entrapment tagging approaches have highlighted their utilization in functional genomics studies in rice. We have also summarised different forward and reverse genetics approaches used for screening of insertional mutant populations. Furthermore, we have compiled information from several efforts made using insertional mutagenesis approaches in rice. The information presented here would serve as a database for rice insertional mutagenesis populations. We have also included various examples which illustrate how these populations have been useful for rice functional genomics studies. The information provided here will be very helpful for future functional genomics studies in rice aimed at its genetic improvement.
插入诱变是利用外源DNA作为诱变剂产生突变群体的一种不可或缺的工具。下一代测序平台的发展使得对产生的突变群体进行更快的筛选和分析成为可能。水稻是世界上一半以上人口的主要主食作物；然而，其基因组中大多数基因的功能尚未得到分析。各种突变群体是实现这一目标的极其宝贵的资源。在这里，我们综述了在水稻中使用的不同插入诱变方法，并讨论了它们的原理、优点和局限性。转移DNA（T-DNA）、转座子和捕获标签方法之间的比较突出了它们在水稻功能基因组学研究中的应用。我们还总结了用于筛选插入突变群体的不同正向和反向遗传学方法。此外，我们汇编了几项利用水稻插入诱变方法所做工作的信息。这里提供的信息将作为水稻插入诱变群体的数据库。我们还列举了各种实例来说明这些群体如何有助于水稻功能基因组学研究。这里提供的信息将对未来旨在改良水稻遗传特性的功能基因组学研究非常有帮助。
