Laboratory Medicine & Pathobiology, University of Toronto, Toronto, ON, Canada.
Public Health Ontario Laboratory, Public Health Ontario, Toronto, ON, Canada.
Sci Rep. 2019 Aug 30;9(1):12615. doi: 10.1038/s41598-019-47740-1.
In 2017 Ontario experienced the largest mumps outbreak in the province in 8 years, at a time when multiple outbreaks were occurring across North America. Of 259 reported cases, 143 occurred in Toronto, primarily among young adults. Routine genotyping of the small hydrophobic gene indicated that the outbreak was due to mumps virus genotype G. We performed a retrospective study of whole genome sequencing of 26 mumps virus isolates from early in the outbreak, using a tiling amplicon method. Results indicated that two of the cases were genetically divergent, with the remaining 24 cases belonging to two major clades and one minor clade. Phylogeographic analysis confirmed circulation of virus from each clade between Toronto and other regions in Ontario. Comparison with other genotype G strains from North America suggested that the presence of co-circulating major clades may have been due to separate importation events from outbreaks in the United States. A transmission network analysis performed with the software program TransPhylo was compared with previously collected epidemiological data. The transmission tree correlated with known epidemiological links between nine patients and identified new potential clusters with no known epidemiological links.
2017 年，安大略省经历了 8 年来省内最大的腮腺炎爆发，此时北美多地也出现了多起爆发。在报告的 259 例病例中，143 例发生在多伦多，主要是年轻人。对小疏水基因的常规基因分型表明，此次爆发是由腮腺炎病毒基因型 G 引起的。我们使用平铺扩增子方法对疫情早期的 26 株腮腺炎病毒分离株进行了全基因组测序的回顾性研究。结果表明，有两例病例具有遗传差异，其余 24 例属于两个主要分支和一个次要分支。系统地理学分析证实了来自每个分支的病毒在多伦多和安大略省其他地区之间的传播。与北美的其他基因型 G 株的比较表明，主要分支的共同循环可能是由于与美国的爆发分别有进口事件引起的。使用 TransPhylo 软件程序进行的传播网络分析与之前收集的流行病学数据进行了比较。传播树与已知的 9 名患者之间的流行病学联系相关，并确定了具有未知流行病学联系的新的潜在集群。
