Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology (Qingdao), No. 1 Wenhai Road, Qingdao 266237, China.
Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology (Qingdao), No. 1 Wenhai Road, Qingdao 266237, China.
Carbohydr Polym. 2019 Nov 15;224:115155. doi: 10.1016/j.carbpol.2019.115155. Epub 2019 Aug 1.
Plant-parasitic nematodes cause substantial crop losses annually; however, current nematicides are environmentally unfriendly and highly toxic to nontarget organisms. The development of green efficient nematicides from multifunctional natural bioactive substances such as chitin oligosaccharide (COS) is promising. In this paper, COS dithiocarbamate derivatives (COSDTC, COSDTA, COSDTB) were synthesized to increase nematicidal activity (against Meloidogyne incognita), and their structures were characterized by FTIR, NMR, TGA/DTG and elemental analysis. Furthermore, the nematicidal activities, egg hatching inhibitory activities, plant growth adjustment abilities, cytotoxicity and phytotoxicity of the derivatives were evaluated. The primary mechanism was assessed by heavy metal ion absorption and GSH-binding assays. The results showed COS dithiocarbamate derivatives could possess multiple efficacies, including high nematicidal activities and egg hatching inhibitory activities, plant growth regulating effects, low cell toxicities and phytotoxicities. Additionally, it was inferred that nematicidal activity may be correlated with GSH-binding activity but not heavy metal ion complexation. COS modification has immense potential for controlling plant-parasitic nematodes.
植物寄生线虫每年都会导致大量作物减产；然而，目前的杀线虫剂对环境不友好，对非目标生物具有高度毒性。从多功能天然生物活性物质（如壳寡糖 (COS)）开发绿色高效杀线虫剂具有广阔的前景。在本文中，合成了壳寡糖二硫代氨基甲酸酯衍生物（COSDTC、COSDTA、COSDTB）以提高杀线虫活性（对南方根结线虫），并通过 FTIR、NMR、TGA/DTG 和元素分析对其结构进行了表征。此外，还评估了衍生物的杀线虫活性、卵孵化抑制活性、植物生长调节能力、细胞毒性和植物毒性。通过重金属离子吸收和 GSH 结合测定评估了主要机制。结果表明，壳寡糖二硫代氨基甲酸酯衍生物具有多种功效，包括高杀线虫活性和卵孵化抑制活性、植物生长调节作用、低细胞毒性和植物毒性。此外，还推断杀线虫活性可能与 GSH 结合活性有关，而与重金属离子络合无关。COS 修饰在防治植物寄生线虫方面具有巨大潜力。
