Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao 266071, China.
Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology (Qingdao), No. 1 Wenhai Road, Qingdao 266237, China.
Mar Drugs. 2020 May 22;18(5):273. doi: 10.3390/md18050273.
Plant root-knot nematode disease is a great agricultural problem and commercially available nematicides have the disadvantages of high toxicity and limited usage; thus, it is urgent to develop new nematicides derived from nature substances. In this study, a novel fluorinated derivative was synthesized by modifying chitosan oligosaccharide (COS) using the strategy of multiple functions. The derivatives were characterized by FTIR, NMR, elemental analysis, and TG/DTG. The activity assays show that the derivatives can effectively kill the second instar larvae of in vitro, among them, chitosan-thiadiazole-trifluorobutene (COSSZFB) perform high eggs hatching inhibitory activity. The derivatives can regulate plant growth (photosynthetic pigment), improve immunity (chitinase and β-1,3-glucanase), and show low cytotoxicity and phytotoxicity. According to the multi-functional activity, the derivatives exhibit a good control effect on plant root-knot nematode disease . The results demonstrate that the COS derivatives (especially fluorinated derivative) perform multiple activities and show the potential to be further evaluated as nematicides.
植物根结线虫病是一个严重的农业问题，市售杀线虫剂存在毒性高、使用受限等缺点；因此，迫切需要开发源自天然物质的新型杀线虫剂。在本研究中，通过使用多功能策略修饰壳聚糖寡糖（COS）合成了一种新型的氟化衍生物。通过 FTIR、NMR、元素分析和 TG/DTG 对衍生物进行了表征。活性测定表明，衍生物能够有效杀死体外的第二龄幼虫，其中壳聚糖-噻二唑-三氟丁烯（COSSZFB）表现出高的卵孵化抑制活性。衍生物可以调节植物生长（光合色素），提高免疫力（几丁质酶和β-1,3-葡聚糖酶），且表现出低细胞毒性和低植物毒性。根据多功能活性，衍生物对植物根结线虫病表现出良好的防治效果。结果表明，COS 衍生物（尤其是氟化衍生物）具有多种活性，有望进一步评估作为杀线虫剂的潜力。
