University of Mazandaran, Faculty of Chemistry, Department of Organic-Polymer Chemistry, Babolsar 47416, Iran.
Cellular and Molecular Biology Research Center (CMBRC), Health Research Institute, Babol University of Medical Sciences, Babol, Iran; Department of Pharmacology, School of Medicine, Babol University of Medical Sciences, Babol, Iran.
Carbohydr Polym. 2019 Nov 15;224:115163. doi: 10.1016/j.carbpol.2019.115163. Epub 2019 Aug 8.
Efficient procedure for preparation of the novel magnetic nanobiocomposite (MNbC) was successfully established by employing click chemistry protocol. The resulted structure exhibited valuable characteristics from biological viewpoints. The prepared materials and obtained nanobiocomposite were characterized by means of nuclear magnetic resonance (NMR), fourier transform infrared (FT-IR), thermal gravimetric analysis (TGA) and differential scanning calorimetry (DSC). To assay the antiproliferative effects of the MNbC; as cancerous cells, Hela, MCF-7 and Saos were evaluated by means of MTT assay and compared with normal fibroblast cell line. Promising antiproliferative effects were observed on both epitheloid and mesenchymal cancer cell lines depending on MNbC concentration and time. To detect the apoptosis-associated changes of cell membranes during the process of apoptosis, Acridine Orange/Ethidium Bromide (AO/EB) dual staining fluorescent technique was incorporated. The studies in this article including all of these specifications represent convincing key findings that make MNbC as an appreciable candidate for further bio-applications.
通过点击化学法成功建立了一种新型的磁性纳米生物复合材料（MNbC）的高效制备方法。该结构从生物学角度表现出了有价值的特性。采用核磁共振（NMR）、傅里叶变换红外（FT-IR）、热重分析（TGA）和差示扫描量热法（DSC）对所制备的材料和获得的纳米生物复合材料进行了表征。为了评估 MNbC 的抗增殖作用，通过 MTT 法对 Hela、MCF-7 和 Saos 等癌细胞系进行了评估，并与正常成纤维细胞系进行了比较。根据 MNbC 的浓度和时间，在两种上皮样和间充质癌细胞系中均观察到了有希望的抗增殖作用。为了检测细胞凋亡过程中细胞膜的凋亡相关变化，采用吖啶橙/溴化乙锭（AO/EB）双重染色荧光技术。本文的研究包括所有这些特性，代表了令人信服的关键发现，使 MNbC 成为进一步生物应用的有价值的候选物。
