Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland.
Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland.
Biochim Biophys Acta Mol Cell Res. 2019 Dec;1866(12):118540. doi: 10.1016/j.bbamcr.2019.118540. Epub 2019 Aug 29.
Acyl carrier protein (ACP) is a principal partner in the cytosolic and mitochondrial fatty acid synthesis (FAS) pathways. The active form holo-ACP serves as FAS platform, using its 4'-phosphopantetheine group to present covalently attached FAS intermediates to the enzymes responsible for the acyl chain elongation process. Mitochondrial unacylated holo-ACP is a component of mammalian mitoribosomes, and acylated ACP species participate as interaction partners in several ACP-LYRM (leucine-tyrosine-arginine motif)-protein heterodimers that act either as assembly factors or subunits of the electron transport chain and Fe-S cluster assembly complexes. Moreover, octanoyl-ACP provides the C8 backbone for endogenous lipoic acid synthesis. Accumulating evidence suggests that mtFAS-generated acyl-ACPs act as signaling molecules in an intramitochondrial metabolic state sensing circuit, coordinating mitochondrial acetyl-CoA levels with mitochondrial respiration, Fe-S cluster biogenesis and protein lipoylation.
酰基辅酶 A 蛋白 (ACP) 是细胞质和线粒体脂肪酸合成 (FAS) 途径中的主要合作伙伴。其活性形式全酶 ACP 作为 FAS 平台，利用其 4′-磷酸泛酰巯基乙胺基团将共价连接的 FAS 中间产物呈现给负责酰基链延伸过程的酶。线粒体未酰化的全酶 ACP 是哺乳动物线粒体核糖体的组成部分，并且酰化的 ACP 物种作为几种 ACP-LYRM（亮氨酸-酪氨酸-精氨酸基序）-蛋白异二聚体的相互作用伙伴参与，这些异二聚体要么作为组装因子，要么作为电子传递链和 Fe-S 簇组装复合物的亚基。此外，辛酰-ACP 为内源性硫辛酸合成提供 C8 骨架。越来越多的证据表明，mtFAS 生成的酰基辅酶 ACP 作为一种信号分子，在一种线粒体代谢状态感应回路中发挥作用，协调线粒体乙酰辅酶 A 水平与线粒体呼吸、Fe-S 簇生物发生和蛋白质硫辛酰化。
