Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84132, USA.
Department of Cell Biology, Harvard University School of Medicine, Boston, MA 02115, USA.
Mol Cell. 2018 Aug 16;71(4):567-580.e4. doi: 10.1016/j.molcel.2018.06.039.
The electron transport chain (ETC) is an important participant in cellular energy conversion, but its biogenesis presents the cell with numerous challenges. To address these complexities, the cell utilizes ETC assembly factors, which include the LYR protein family. Each member of this family interacts with the mitochondrial acyl carrier protein (ACP), the scaffold protein upon which the mitochondrial fatty acid synthesis (mtFAS) pathway builds fatty acyl chains from acetyl-CoA. We demonstrate that the acylated form of ACP is an acetyl-CoA-dependent allosteric activator of the LYR protein family used to stimulate ETC biogenesis. By tuning ETC assembly to the abundance of acetyl-CoA, which is the major fuel of the TCA cycle and ETC, this system could provide an elegant mechanism for coordinating the assembly of ETC complexes with one another and with substrate availability.
电子传递链（ETC）是细胞能量转换的重要参与者，但它的生物发生给细胞带来了许多挑战。为了解决这些复杂性，细胞利用 ETC 组装因子，其中包括 LYR 蛋白家族。该家族的每个成员都与线粒体酰基辅酶 A 载体蛋白（ACP）相互作用，ACP 是线粒体脂肪酸合成（mtFAS）途径从乙酰辅酶 A 构建脂肪酸链的支架蛋白。我们证明 ACP 的酰化形式是乙酰辅酶 A 依赖性的 LYR 蛋白家族的别构激活剂，用于刺激 ETC 的生物发生。通过将 ETC 组装调谐到乙酰辅酶 A 的丰度，乙酰辅酶 A 是 TCA 循环和 ETC 的主要燃料，这个系统可以为协调 ETC 复合物彼此之间以及与底物可用性的组装提供一个优雅的机制。
