School of Food Science and Engineering, South China University of Technology, No. 381 Wushan Road, Guangzhou 510640, China.
School of Food Science and Engineering, South China University of Technology, No. 381 Wushan Road, Guangzhou 510640, China; Innovation Center of Bioactive Molecule Development and Application, South China Institute of Collaborative Innovation, Xuefu Road, Dongguan 221116, China.
Int J Biol Macromol. 2019 Dec 1;141:460-470. doi: 10.1016/j.ijbiomac.2019.08.238. Epub 2019 Aug 29.
Based on the concept of endophytic fungus, if endophytic fungus can produce the same or similar product as the host plant, which will get rid of the restrictions of farmland, seasonal and pest, the active product could be sustainably obtained. In this study, an endophytic fungus polysaccharide FP showing the similar structure with the host Dendrobium officinale polysaccharide (DOP) was sustainably and cost-effectively obtained under preferred reaction conditions with different C/N ratio. The FP with high yield up to 2.77 ± 0.51 g/L showed same monosaccharide composition with DOP as well as some host-plant-associated polysaccharides in published literatures. The main chain of FP was composed by →3,6)-β-L-Man-(1→, α-D-Glc-(1→, →4)-α-D-Glc-(1→, →3,6)-β-D-Gal-(1→, and →6)-β-D-Gal-(1→, while the side chain was α-D-Glc-(1→. Meanwhile, FP was confirmed as a safe polysaccharide with good antioxidant, antiglycation and immunomodulatory activities. Furthermore, TLR2 and TLR4 were confirmed as the membrane receptors of FP on RAW264.7 cells.
基于内共生真菌的概念，如果内共生真菌能够产生与宿主植物相同或相似的产物，那么它将摆脱农田、季节和害虫的限制，可以可持续地获得活性产物。在这项研究中，在不同 C/N 比的优选反应条件下，可持续且经济高效地获得了一种与铁皮石斛多糖（DOP）结构相似的内共生真菌多糖 FP。FP 的产量高达 2.77±0.51 g/L，其单糖组成与 DOP 以及已发表文献中的一些与宿主植物相关的多糖相同。FP 的主链由 →3,6)-β-L-Man-(1→, α-D-Glc-(1→, →4)-α-D-Glc-(1→, →3,6)-β-D-Gal-(1→ 和 →6)-β-D-Gal-(1→ 组成，而侧链为 α-D-Glc-(1→。同时，FP 被确认为一种安全的多糖，具有良好的抗氧化、抗糖化和免疫调节活性。此外，TLR2 和 TLR4 被确认为 FP 在 RAW264.7 细胞上的膜受体。
