Department of Bio-Health Convergence, Kangwon National University, Chuncheon, 200-701, South Korea.
Chuncheon Center, Korea Basic Science Institute (KBSI), Chuncheon, 24341, Republic of Korea.
Appl Biochem Biotechnol. 2023 Jun;195(6):3822-3839. doi: 10.1007/s12010-022-04187-x. Epub 2022 Oct 19.
In this study, two extracellular polysaccharides (TEPS1 and TEPS2) were isolated from the endophytic fungus (Talaromyces purpureogenus) and purified by DEAE-Sepharose Fast Flow column using NaCl as gradient eluent. The HPLC analysis displayed that TEPS1 was composed of mannose (38.70%), ribose (25.02%), glucose (19.34%), and galactose (16.94%) while the TEPS2 composed by mannose (100%). The NMR results indicated that TEPS1 exhibited α-glycosidic configurations. The both polysaccharides, TEPS1 and TEPS2 were exhibited a good antioxidant activity in terms of DPPH, ABTS, and •OH scavenging. However, TEPS1 showed a higher antioxidant activity than TEPS2. The IC of TEPS1 were 32.16, 192.57, and 54.67 μg·mL, for DPPH, ABTS, and •OH radical scavenging, respectively. Furthermore, TEPS1 showed the high cellular antioxidant and wound healing activity in the human embryonic kidney (HEK293) cell line. Overall, these two polysaccharides were promising in antioxidant activity.
在这项研究中，从内生真菌（紫色红曲菌）中分离得到两种胞外多糖（TEPS1 和 TEPS2），并使用 DEAE-Sepharose Fast Flow 柱，以 NaCl 为梯度洗脱剂进行纯化。HPLC 分析表明，TEPS1 由甘露糖（38.70%）、核糖（25.02%）、葡萄糖（19.34%）和半乳糖（16.94%）组成，而 TEPS2 则由甘露糖（100%）组成。NMR 结果表明，TEPS1 呈现α-糖苷键构型。两种多糖，TEPS1 和 TEPS2 在 DPPH、ABTS 和 •OH 清除方面均表现出良好的抗氧化活性。然而，TEPS1 的抗氧化活性高于 TEPS2。TEPS1 的 IC 分别为 32.16、192.57 和 54.67μg·mL，用于 DPPH、ABTS 和 •OH 自由基清除。此外，TEPS1 在人胚肾（HEK293）细胞系中表现出较高的细胞抗氧化和伤口愈合活性。总体而言，这两种多糖在抗氧化活性方面具有很大的潜力。
