Bader Robert, Ibrahim José-Noel, Mourad Ali, Moussa Mayssam, Azoury Joan, Azoury Joseph, Alaaeddine Nada
Regenerative Medicine and Inflammation Laboratory, Faculty of Medicine, Saint-Joseph University, Beirut, Lebanon.
Faculty of Public Health II, Lebanese University, Beirut, Lebanon.
Int J Stem Cells. 2019 Nov 30;12(3):388-399. doi: 10.15283/ijsc19047.
Oxidative stress (OS) is known to be an important factor of male infertility. Adipose-derived mesenchymal stem cells (AD-MSCs) are known to have immune-modulatory and anti-oxidant effects through their secretions, hence raising the idea of their potential benefit to improve sperm parameters. This study aims at investigating the effect of AD-MSCs conditioned medium (CM) on human sperm parameters in the presence and absence of HO-induced OS.
Sperm samples were collected from 30 healthy men and divided into two groups: non-stressed and HO-stressed. Isolated AD-MSCs from healthy donors undergoing liposuction were cultured and CM was collected at 24, 48 and 72 h. Both sperm groups were cultured with CM and a time course was performed followed by an evaluation of sperm parameters. The incubation of non-stressed and stressed sperm samples with AD-MSCs-CM for 24 h was found to have the optimum impact on sperm vacuolization, DNA fragmentation and OS levels in comparison to other incubation timings, while preserving motility, viability and morphology of cells. Incubation with CM improved all sperm parameters except morphology in comparison to the non-treated group, with the best effect noted with CM collected at 24 h rather than 48 or 72 h for sperm vacuolization and DNA fragmentation. When compared to fresh semen parameters (T0), samples cultured with CM 24 h showed a significant decrease in sperm vacuolization and DNA fragmentation while keeping other parameters stable.
AD-MSCSs-CM improves sperm quality, and hence can be used in treating infertility and subsequently enhancing IVF outcomes.
氧化应激（OS）是男性不育的一个重要因素。脂肪来源的间充质干细胞（AD-MSCs）已知通过其分泌产物具有免疫调节和抗氧化作用，因此引发了其对改善精子参数可能有益的想法。本研究旨在调查AD-MSCs条件培养基（CM）在有和没有过氧化氢（HO）诱导的氧化应激情况下对人类精子参数的影响。
从30名健康男性收集精子样本并分为两组：非应激组和HO应激组。从接受抽脂术的健康供体中分离出AD-MSCs进行培养，并在24、48和72小时收集CM。两组精子均与CM一起培养，并进行时间进程观察，随后评估精子参数。与其他孵育时间相比，发现非应激和应激精子样本与AD-MSCs-CM孵育24小时对精子空泡化、DNA片段化和氧化应激水平具有最佳影响，同时保持细胞的活力、生存力和形态。与未处理组相比，与CM孵育改善了除形态外的所有精子参数，对于精子空泡化和DNA片段化，24小时收集的CM效果最佳，而非采集48或72小时的CM。与新鲜精液参数（T0）相比，用CM培养24小时的样本精子空泡化和DNA片段化显著降低，同时其他参数保持稳定。
AD-MSCs-CM可改善精子质量，因此可用于治疗不育症并随后提高体外受精（IVF）成功率。
