Department of Biomedical Engineering, The Ohio State University, Columbus, Ohio, USA.
Biophysics Graduate Program, The Ohio State University, Columbus, Ohio, USA.
Curr Eye Res. 2020 Feb;45(2):111-117. doi: 10.1080/02713683.2019.1663387. Epub 2019 Sep 18.
: The purpose of this study was to develop an effective treatment method using poloxamers to restore and maintain physiological hydration in postmortem porcine and human corneas during ex vivo experimentation, and to compare corneal inflation response with or without treatment.: Corneal buttons obtained from whole globes (n = 30 porcine, n = 8 human) were treated with various concentrations of poloxamer 188 (P188, a synthetic macromolecule surfactant) for 24 hrs to identify the concentration that would return the cornea to near-physiological hydration (i.e. H = 3.2). Whole globes (n = 12 porcine, n = 16 human) were also used to monitor central corneal thickness (CCT) during deswelling treatment. Inflation testing from 5 to 30 mmHg was performed in the porcine globes and a subset of human globes to characterize the mechanical response of the cornea after treatment.: Physiological hydration was obtained after 24 hrs immersion in 3.25% P188 for porcine corneas and 4.25% P188 treatment for human corneas. CCT was stabilized and returned to physiological levels after 24 hrs of treatment in 3.25% P188 in porcine (891 ± 66 µm) and 4.25% P188 in human (574 ± 34 µm) whole globes. Corneal axial strains at 30 mmHg were significantly larger at physiological hydration than in swollen cornea in both porcine (-6.42%±1.50% vs. -3.64%±1.05%, = .004) and human (-2.85%±0.09% in vs. -1.53%±0.27%, = .031) eyes.: Our results suggest that P188 treatment was effective in restoring and maintaining near physiological corneal hydration during ex vivo testing, and hydration appeared to significantly impact corneal inflation response in both porcine and human eyes.
本研究旨在开发一种使用泊洛沙姆的有效治疗方法，以在离体实验中恢复和维持死后猪和人眼角膜的生理水合作用，并比较有或没有治疗的角膜膨胀反应。从整个眼球（n = 30 只猪，n = 8 人）获得的角膜瓣用不同浓度的泊洛沙姆 188（P188，一种合成大分子表面活性剂）处理 24 小时，以确定使角膜恢复接近生理水合作用的浓度（即 H = 3.2）。还使用整个眼球（n = 12 只猪，n = 16 人）监测去水肿治疗过程中的中央角膜厚度（CCT）。在猪眼球和部分人眼球中进行 5 至 30 毫米汞柱的膨胀测试，以表征治疗后角膜的机械响应。在猪角膜中，经过 24 小时 3.25% P188 浸泡后获得生理水合作用，在人角膜中，经过 24 小时 4.25% P188 处理后获得生理水合作用。在猪（891 ± 66 µm）和人（574 ± 34 µm）整个眼球中，用 3.25% P188 治疗 24 小时后，CCT 稳定并恢复至生理水平。在生理水合作用下，猪（-6.42%±1.50% 对-3.64%±1.05%， = 0.004）和人（-2.85%±0.09% 对-1.53%±0.27%， = 0.031）眼角膜在 30 毫米汞柱时的轴向应变均显著大于肿胀眼角膜。：我们的结果表明，P188 治疗在离体测试中有效恢复和维持了接近生理的角膜水合作用，水合作用似乎显著影响了猪和人眼角膜的膨胀反应。
