Department of Chemistry, Federal Urdu University of Arts, Science and Technology, Gulshan-e-Iqbal, Campus Karachi, Karachi, Pakistan.
Department of Chemistry, Federal Urdu University of Arts, Science and Technology, Gulshan-e-Iqbal, Campus Karachi, Karachi, Pakistan; H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Jan 15;225:117489. doi: 10.1016/j.saa.2019.117489. Epub 2019 Aug 27.
The monitoring of residual antibiotics in the environment has gained a significant importance for the effective control, because of the high risk to human health. A simple strategy was designed for the green synthesis and detection of doxycycline (Dox) by using anionic surfactant sodium bis 2-ethylhexylsulfosuccinate based silver nanoparticles (AOT-AgNPs). The chemical reduction and capping of Ag ions was achieved by sulfonyl and carbonyl functional groups of AOT molecule. The AOT-AgNPs were found to have excellent stability at variable environmental parameters (i.e. temperature, storage period, salt concentration and pH) possibly due to the strong emulsifying nature of the surfactant. Mechanism of interaction between the AOT-AgNPs and Dox was established with UV/visible, Fourier transform infrared (FTIR) spectroscopy, Atomic force microscopy (AFM) and Dynamic light scattering (DLS) techniques, which suggests the interaction via aggregates formation. The synthesize probe could detect the Dox within 15 min over a wide range of concentrations from 0.1 to 140μM with limit of detection (LOD) of 0.2 μM. As proof of strategy, we have illustrated that the AOT-AgNPs also detect Dox in biological and environmental samples with negligible interference and very significant recovery rates. Moreover, non-toxic nature against various tested cell lines (i.e. normal mouse fibroblast (NIH-3 T3) and cancerous non-small lung carcinoma (NCI-H460)) and significant antimicrobial, antibiofilm and biofilm eradicating potential of AOT-AgNPs were provide ideal nanomaterial for further applications.
环境中残留抗生素的监测对于有效控制具有重要意义，因为其对人类健康存在高风险。本研究设计了一种简单的策略，通过使用阴离子表面活性剂双(2-乙基己基)磺基琥珀酸钠（AOT）基银纳米粒子（AOT-AgNPs）进行绿色合成和检测强力霉素（Dox）。AOT 分子的磺酰基和羰基官能团实现了 Ag 离子的化学还原和封端。AOT-AgNPs 在各种环境参数（即温度、储存期、盐浓度和 pH 值）下表现出优异的稳定性，这可能是由于表面活性剂的强乳化性质。通过紫外/可见分光光度法、傅里叶变换红外（FTIR）光谱、原子力显微镜（AFM）和动态光散射（DLS）技术建立了 AOT-AgNPs 与 Dox 之间的相互作用机制，表明通过聚集物形成发生相互作用。该合成探针可以在 0.1 至 140μM 的宽浓度范围内在 15 分钟内检测到 Dox，检测限（LOD）为 0.2μM。作为策略的证明，我们已经说明了 AOT-AgNPs 还可以在生物和环境样品中检测 Dox，几乎没有干扰，并且具有非常高的回收率。此外，AOT-AgNPs 对各种测试细胞系（即正常小鼠成纤维细胞（NIH-3T3）和非小细胞肺癌（NCI-H460））具有无毒性质，并且具有显著的抗菌、抗生物膜和生物膜消除潜力，为进一步应用提供了理想的纳米材料。
