School of Chemistry, Physics and Mechanical Engineering (CPME), Science and Engineering Faculty (SEF), Institute of Health and Biomedical Innovation (IHBI), Queensland University of Technology (QUT), Brisbane, Australia. Doran Laboratory, School of Biomedical Sciences, Faculty of Health, Institute of Health and Biomedical Innovation (IHBI), Queensland University of Technology (QUT), Brisbane, Australia. Translational Research Institute (TRI), Brisbane, Australia.
Biofabrication. 2019 Dec 4;12(1):015015. doi: 10.1088/1758-5090/ab4053.
We aimed to capture the outstanding mechanical properties of meshes, manufactured using textile technologies, in thin biodegradable biphasic tissue-engineered scaffolds through encapsulation of meshes into porous structures formed from the same polymer. Our novel manufacturing process used thermally induced phase separation (TIPS), with ethylene carbonate (EC) as the solvent, to encapsulate a poly(lactic-co-glycolic acid) (PLGA) mesh into a porous PLGA network. Biphasic scaffolds (1 cm × 4 cm × 300 μm) were manufactured by immersing strips of PLGA mesh in 40 °C solutions containing 5% PLGA in EC, supercooling at 4 °C for 4 min, triggering TIPS by manually agitating the supercooled solution, and lastly eluting EC into 4 °C Milli-Q water. EC processing was rapid and did not compromise mesh tensile properties. Biphasic scaffolds exhibited a tensile strength of 40.7 ± 2.2 MPa, porosity of 94%, pore size of 16.85 ± 3.78 μm, supported HaCaT cell proliferation, and degraded in vitro linearly over the first ∼3 weeks followed by rapid degradation over the following three weeks. The successful integration of textile-type meshes yielded scaffolds with exceptional mechanical properties. This thin, porous, high-strength scaffold is potentially suitable for use in dermal wound repair or repair of tubular organs.
我们旨在通过将网格封装到由相同聚合物形成的多孔结构中，在薄的可生物降解双相组织工程支架中捕捉到使用纺织技术制造的网格的出色机械性能。我们的新型制造工艺使用热致相分离（TIPS），以碳酸亚乙酯（EC）为溶剂，将聚（乳酸-共-乙醇酸）（PLGA）网格封装到多孔 PLGA 网络中。通过将 PLGA 网格条浸入含有 5%PLGA 的 EC 40°C 溶液中，在 4°C 下超冷 4 分钟，手动搅拌超冷溶液触发 TIPS，最后将 EC 洗脱到 4°C 的 Milli-Q 水中，制造出双相支架（1cm×4cm×300μm）。EC 处理速度快，不会影响网格的拉伸性能。双相支架的拉伸强度为 40.7±2.2MPa，孔隙率为 94%，孔径为 16.85±3.78μm，支持 HaCaT 细胞增殖，并在体外线性降解，前 3 周内迅速降解。纺织型网格的成功集成产生了具有出色机械性能的支架。这种薄、多孔、高强度的支架可能适用于皮肤伤口修复或管状器官修复。
