Nguyen Cong Oanh, Taminiau Bernard, Pham Kim Dang, Daube Georges, Nguyen Van Giap, Bindelle Jérôme, Abdulaye Fall Papa, Vu Dinh Ton, Hornick Jean-Luc
Department of Veterinary Management of Animal Resources, FARAH Center, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, Belgium.
Faculty of Animal Science, Vietnam National University of Agriculture, Hanoi Capital 100000, Vietnam.
Asian-Australas J Anim Sci. 2020 May;33(5):788-801. doi: 10.5713/ajas.19.0278. Epub 2019 Aug 3.
This study was conducted to evaluate the effects of diets containing different wet rice distillers' by-product (RDP) levels on growth performance, nutrient digestibility, blood profiles and gut microbiome of weaned piglets.
A total of 48 weaned castrated male crossbred pigs, initial body weight 7.54±0.97 kg, and age about 4 wks, were used in this experiment. The piglets were randomly allocated into three iso-nitrogenous diet groups that were fed either a control diet, a diet with 15% RDP, or a diet with 30% RDP for a total of 35 days. Chromium oxide was used for apparent digestibility measurements. On d 14 and d 35, half of the piglets were randomly selected for hemato-biochemical and gut microbiota evaluations.
Increasing inclusion levels of RDP tended to linearly increase (p≤0.07) average daily gain on d 14 and d 35, and decreased (p = 0.08) feed conversion ratio on d 35. Empty stomach weight increased (p = 0.03) on d 35 while digestibility of diet components decreased. Serum globulin concentration decreased on d 14 (p = 0.003) and red blood cell count tended to decrease (p = 0.06) on d 35, parallel to increase RDP levels. Gene amplicon profiling of 16S rRNA revealed that the colonic microbiota composition of weaned pigs changed by inclusion of RDP over the period. On d 14, decreased proportions of Lachnospiraceae_ge, Ruminococcaceae_ge, Ruminococcaceae_UCG-005, and Bacteroidales_ge, and increased proportions of Prevotellaceae_ge, Prevotella_2, and Prevotella_9 were found with inclusion of RDP, whereas opposite effect was found on d 35. Additionally, the proportion of Lachnospiraceae_ge, Ruminococcaceae_ge, Ruminococcaceae_UCG-005, and Bacteroidales_ge in RDP diets decreased over periods in control diet but increased largely in diet with 30% RDP.
These results indicate that RDP in a favorable way modulate gastrointestinal microbiota composition and improve piglet performance despite a negative impact on digestibility of lipids and gross energy.
本研究旨在评估含有不同水平湿稻谷酒糟副产物（RDP）的日粮对断奶仔猪生长性能、养分消化率、血液指标和肠道微生物群的影响。
本试验选用48头断奶去势雄性杂交仔猪，初始体重7.54±0.97 kg，年龄约4周。仔猪被随机分为三个等氮日粮组，分别饲喂对照日粮、含15%RDP的日粮或含30%RDP的日粮，共35天。使用氧化铬进行表观消化率测定。在第14天和第35天，随机选择一半仔猪进行血液生化和肠道微生物群评估。
RDP添加水平的增加在第14天和第35天倾向于使平均日增重呈线性增加（p≤0.07），并在第35天降低了饲料转化率（p = 0.08）。第35天空腹体重增加（p = 0.03），而日粮成分的消化率降低。第14天血清球蛋白浓度降低（p = 0.003），第35天红细胞计数倾向于降低（p = 0.06），与RDP水平的增加平行。16S rRNA基因扩增子分析表明，在此期间，日粮中添加RDP改变了断奶仔猪的结肠微生物群组成。在第14天，日粮中添加RDP后，毛螺菌科_ge、瘤胃球菌科_ge、瘤胃球菌科_UCG - 005和拟杆菌目_ge的比例降低，普雷沃氏菌科_ge、普雷沃氏菌属_2和普雷沃氏菌属_9的比例增加，而在第35天则发现相反的效果。此外，对照日粮中RDP日粮中毛螺菌科_ge、瘤胃球菌科_ge、瘤胃球菌科_UCG - 005和拟杆菌目_ge的比例随时间下降，但在含30%RDP的日粮中大幅增加。
这些结果表明，尽管RDP对脂质和总能的消化率有负面影响，但它以有益的方式调节胃肠道微生物群组成并改善仔猪性能。
