Fetz Allison E, Fantaziu Cristina A, Smith Richard A, Radic Marko Z, Bowlin Gary L
Department of Biomedical Engineering, University of Memphis, Memphis, TN 38152, USA.
Department of Biomedical Engineering, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA.
Bioengineering (Basel). 2019 Aug 31;6(3):78. doi: 10.3390/bioengineering6030078.
Neutrophils, the first cells that interact with surface-adsorbed proteins on biomaterials, have been increasingly recognized as critical maestros in the foreign body response for guided tissue regeneration. Recent research has shown that small diameter (SD) fibers of electrospun tissue regeneration templates, which have a high surface area to volume ratio (SAVR), enhance the release of neutrophil extracellular traps (NETs) compared to large diameter (LD) fibers, resulting in impaired tissue regeneration. In this study, we evaluated the adsorption of eight human serum proteins on the surface of electrospun templates to investigate how protein adsorption may regulate the release of NETs. Electrospun polydioxanone templates made from SD fibers with high SAVR and LD fibers with low SAVR, were incubated with 0.2% human serum and in situ protein adsorption was quantified with infrared-based immunodetection. Of the detected proteins, IgM and vitronectin adsorbed at low levels, suggesting that they do not play a central role in the release of NETs. Contrastingly, albumin and IgG adsorbed rapidly to the surface of the templates. One-hundred to 200 times more IgG adsorbed on the templates compared to albumin, with significantly greater adsorption occurring on the SD templates with high SAVR. Given that neutrophils express receptors that interact with IgG during phagocytosis and NET release, these results suggest that SAVR-dependent adsorption of IgG on the SD electrospun templates may contribute to the up-regulated release of NETs. Overall, this study may aid in the design of immunomodulatory biomaterials that regulate NET release and thus the potential for neutrophil-driven tissue regeneration.
中性粒细胞是最早与生物材料表面吸附蛋白相互作用的细胞，越来越被认为是引导组织再生的异物反应中的关键指挥者。最近的研究表明，与大直径（LD）纤维相比，具有高表面积与体积比（SAVR）的电纺组织再生模板的小直径（SD）纤维会增强中性粒细胞胞外诱捕网（NETs）的释放，从而导致组织再生受损。在本研究中，我们评估了八种人血清蛋白在电纺模板表面的吸附情况，以研究蛋白吸附如何调节NETs的释放。由具有高SAVR的SD纤维和低SAVR的LD纤维制成的电纺聚二氧六环酮模板，与0.2%的人血清一起孵育，并通过基于红外的免疫检测对原位蛋白吸附进行定量。在检测到的蛋白中，IgM和玻连蛋白的吸附水平较低，表明它们在NETs的释放中不发挥核心作用。相反，白蛋白和IgG迅速吸附到模板表面。与白蛋白相比，吸附在模板上的IgG多100到200倍，在具有高SAVR的SD模板上的吸附明显更多。鉴于中性粒细胞在吞噬作用和NET释放过程中表达与IgG相互作用 的受体，这些结果表明，IgG在SD电纺模板上的SAVR依赖性吸附可能有助于上调NETs的释放。总体而言，本研究可能有助于设计调节NET释放的免疫调节生物材料，从而有助于中性粒细胞驱动的组织再生。
