Vitkov Ljubomir, Krautgartner Wolf-Dietrich, Obermayer Astrid, Stoiber Walter, Hannig Matthias, Klappacher Michaela, Hartl Dominik
Department of Zoological Structure Research, Cell Biology, University of Salzburg, Salzburg, Austria; Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, Saarland University, Homburg, Germany.
Department of Zoological Structure Research, Cell Biology, University of Salzburg, Salzburg, Austria.
PLoS One. 2015 Mar 23;10(3):e0121359. doi: 10.1371/journal.pone.0121359. eCollection 2015.
Implants trigger an inflammatory response, which is important for osseointegration. Here we studied neutrophil extracellular trap (NET) release of human neutrophils in response to sandblasted large-grit acid etched (SLA) implants using fluorescent, confocal laser scanning and scanning electron microscopy. Our studies demonstrate that human neutrophils rapidly adhered to SLA surfaces, which triggered histone citrullination and NET release. Further studies showed that albumin or acetylsalicylic acid had no significant effects on the inflammatory response to SLA surfaces. In contrast to bioinert materials, which do not osseointegrate, the bioactivity of SLA surfaces is coupled with the ability to release NETs. Further investigations are necessary for clarifying the role of NETosis for osseointegration.
植入物会引发炎症反应，这对骨整合很重要。在此，我们使用荧光、共聚焦激光扫描和扫描电子显微镜研究了人中性粒细胞对喷砂大颗粒酸蚀（SLA）植入物的中性粒细胞胞外陷阱（NET）释放情况。我们的研究表明，人中性粒细胞迅速黏附于SLA表面，这引发了组蛋白瓜氨酸化和NET释放。进一步研究表明，白蛋白或乙酰水杨酸对SLA表面的炎症反应无显著影响。与不发生骨整合的生物惰性材料不同，SLA表面的生物活性与释放NETs的能力相关。为阐明NETosis在骨整合中的作用，还需要进一步研究。
