Ghosh Saptarshi, Jassar Ola, Kontsedalov Svetlana, Lebedev Galina, Wang Chunxia, Turner Donielle, Levy Amit, Ghanim Murad
Department of Entomology, the Volcani Center, Rishon LeZion 7505101, Israel.
Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, USA.
Insects. 2019 Sep 2;10(9):279. doi: 10.3390/insects10090279.
Liberibacter solanacerum (CLso), transmitted by in a persistent and propagative mode causes carrot yellows disease, inflicting hefty economic losses. Understanding the process of transmission of CLso by psyllids is fundamental to devise sustainable management strategies. Persistent transmission involves critical steps of adhesion, cell invasion, and replication before passage through the midgut barrier. This study uses a transcriptomic approach for the identification of differentially expressed genes with CLso infection in the midguts, adults, and nymphs of and their putative involvement in CLso transmission. Several genes related to focal adhesion and cellular invasion were upregulated after CLso infection. Interestingly, genes involved with proper functionality of the endoplasmic reticulum (ER) were upregulated in CLso infected samples. Notably, genes from the endoplasmic reticulum associated degradation (ERAD) and the unfolded protein response (UPR) pathway were overexpressed after CLso infection. Marker genes of the ERAD and UPR pathways were also upregulated in when infected with Liberibacter asiaticus (CLas). Upregulation of the ERAD and UPR pathways indicate induction of ER stress by CLso/CLas in their psyllid vector. The role of ER in bacteria-host interactions is well-documented; however, the ER role following pathogenesis of CLso/CLas is unknown and requires further functional validation.
番茄细菌性黄化病菌（CLso）通过木虱以持久增殖的方式传播，会引发胡萝卜黄化病，造成巨大经济损失。了解木虱传播CLso的过程是制定可持续管理策略的基础。持久传播涉及在穿过中肠屏障之前的粘附、细胞入侵和复制等关键步骤。本研究采用转录组学方法，以鉴定在CLso感染的木虱中肠、成虫和若虫中差异表达的基因，以及它们在CLso传播中的假定作用。CLso感染后，几个与粘着斑和细胞入侵相关的基因上调。有趣的是，内质网（ER）正常功能相关的基因在CLso感染的样本中上调。值得注意的是，内质网相关降解（ERAD）和未折叠蛋白反应（UPR）途径的基因在CLso感染后过度表达。当感染亚洲韧皮杆菌（CLas）时，ERAD和UPR途径的标记基因在木虱中也上调。ERAD和UPR途径的上调表明CLso/CLas在其木虱载体中诱导了内质网应激。内质网在细菌与宿主相互作用中的作用已有充分记载；然而，CLso/CLas致病后内质网的作用尚不清楚，需要进一步的功能验证。
