Petrochemistry and Polymer Science, Faculty of Science, Chulalongkorn University, Patumwan, Bangkok 10330, Thailand.
Sensors (Basel). 2013 Sep 26;13(10):13039-53. doi: 10.3390/s131013039.
Ferulic acid is an important phenolic antioxidant found in or added to diet supplements, beverages, and cosmetic creams. Two designs of paper-based platforms for the fast, simple and inexpensive evaluation of ferulic acid contents in food and pharmaceutical cosmetics were evaluated. The first, a paper-based electrochemical device, was developed for ferulic acid detection in uncomplicated matrix samples and was created by the photolithographic method. The second, a paper-based colorimetric device was preceded by thin layer chromatography (TLC) for the separation and detection of ferulic acid in complex samples using a silica plate stationary phase and an 85:15:1 (v/v/v) chloroform: methanol: formic acid mobile phase. After separation, ferulic acid containing section of the TLC plate was attached onto the patterned paper containing the colorimetric reagent and eluted with ethanol. The resulting color change was photographed and quantitatively converted to intensity. Under the optimal conditions, the limit of detection of ferulic acid was found to be 1 ppm and 7 ppm (S/N = 3) for first and second designs, respectively, with good agreement with the standard HPLC-UV detection method. Therefore, these methods can be used for the simple, rapid, inexpensive and sensitive quantification of ferulic acid in a variety of samples.
阿魏酸是一种重要的酚类抗氧化剂，存在于饮食补充剂、饮料和美容霜中，或作为添加剂添加其中。本文评估了两种用于快速、简便、廉价评估食品和药用化妆品中阿魏酸含量的基于纸张的平台设计。第一种是基于纸张的电化学装置，通过光刻法开发用于检测简单基质样品中的阿魏酸。第二种是基于纸张的比色装置，首先通过薄层层析 (TLC) 对复杂样品中的阿魏酸进行分离和检测，使用硅胶板固定相和 85:15:1 (v/v/v) 氯仿:甲醇:甲酸流动相。分离后，将含有阿魏酸的 TLC 板部分粘贴到包含比色试剂的图案化纸张上，并使用乙醇洗脱。拍摄所得颜色变化并将其定量转换为强度。在最佳条件下，两种设计的阿魏酸检测限分别为 1 ppm 和 7 ppm（S/N = 3），与标准 HPLC-UV 检测方法具有良好的一致性。因此，这些方法可用于各种样品中阿魏酸的简单、快速、廉价和灵敏定量。
