Laboratoire d'Ecologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie, Centre Urbain Nord, BP 676, 1080 Tunis, Tunisia.
Laboratoire de Microbiologie M10, UMR 2359, Aix-Marseille Univ., Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
Int J Syst Evol Microbiol. 2012 Jun;62(Pt 6):1365-1368. doi: 10.1099/ijs.0.034603-0. Epub 2011 Aug 5.
Strain BELH1(T), a novel mesophilic, anaerobic, halotolerant, rod-shaped bacterium, was isolated from a Tunisian wastewater digester. The cells of the strain are motile, measure 0.5×2-5 µm, and occur singly or in pairs. The strain reduced thiosulfate and elemental sulfur (but not sulfate or sulfite) into sulfide. It grew at 15-40 °C (optimum 30 °C), pH 5.8-8.4 (optimum 7) and with 0-10 % (w/v) NaCl (optimum 3.0 %). The genomic DNA G+C content of strain BELH1(T) was 38.2 mol% and the strain's predominant cellular fatty acids were C(14:0), a summed feature that contained iso-C(17:1) and/or anteiso-C(17:1) B, and C(16:0). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel strain was most closely related to Fusibacter paucivorans (94.8 % sequence similarity). Based on phenotypic, phylogenetic and taxonomic characteristics, strain BELH1(T) represents a novel species of the genus Fusibacter, for which the name Fusibacter tunisiensis sp. nov. is proposed. The type strain is BELH1(T) ( = DSM 24436(T) = JCM 17481(T)).
菌株 BELH1(T)，一种新型的嗜中温、厌氧、耐盐、杆状细菌，是从突尼斯废水消化器中分离出来的。该菌株的细胞可运动，大小为 0.5×2-5 µm，呈单生或成对出现。该菌株将硫代硫酸盐和元素硫（但不是硫酸盐或亚硫酸盐）还原为硫化物。它在 15-40°C（最佳 30°C）、pH 5.8-8.4（最佳 7）和 0-10%（w/v）NaCl（最佳 3.0%）下生长。菌株 BELH1(T) 的基因组 DNA G+C 含量为 38.2 mol%，其主要细胞脂肪酸为 C(14:0)，这是一个包含 iso-C(17:1) 和/或 anteiso-C(17:1) B 的总和特征，以及 C(16:0)。基于 16S rRNA 基因序列的系统发育分析表明，该新型菌株与 Fusibacter paucivorans（相似度 94.8%）最为密切相关。基于表型、系统发育和分类特征，菌株 BELH1(T) 代表 Fusibacter 属的一个新种，建议将其命名为 Fusibacter tunisiensis sp. nov.。模式菌株是 BELH1(T)（=DSM 24436(T)=JCM 17481(T)）。
