University of Chinese Academy of Sciences, Beijing 100101, PR China.
Key Laboratory of Alpine Ecology, Chinese Academy of Sciences, Beijing 100101, PR China.
Int J Syst Evol Microbiol. 2019 Nov;69(11):3519-3523. doi: 10.1099/ijsem.0.003653.
A Gram-stain-positive, rod-shaped and motile bacterium with lateral flagellum, designated T3246-1, was isolated from an ice core, which was drilled from Hariqin Glacier on the Tibetan Plateau, PR China. It grew optimally at 20 °C, pH 7-8 and in the presence of 3 % (w/v) NaCl. The major fatty acid of strain T3246-1 was anteiso-C. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. MK-8 was the dominant isoprenoid quinone. The whole-cell sugars were rhamnose, xylose and mannose. The major cell-wall peptidoglycan was lysine. The genomic DNA G+C content of the strain was 71.4 mol%. Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain T3246-1 formed a lineage within the genus and was closely related to YIM 93306 with 95.99 % similarity. The average nucleotide identity value between strain T3246-1 and YIM 93306 was 76.65 %. Based on phenotypic and chemotaxonomic characteristics, strain T3246-1 was considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is T3246-1 (=CGMCC 1.13535=JCM 32923).
一株革兰氏阳性、杆状、具有侧鞭毛、可运动的细菌，命名为 T3246-1，是从中国青藏高原哈里钦冰川的冰芯中分离得到的。它在 20°C、pH7-8 和 3%（w/v）NaCl 的条件下生长最佳。菌株 T3246-1 的主要脂肪酸为anteiso-C。主要极性脂为双磷脂酰甘油、磷脂酰甘油和磷脂酰肌醇。MK-8 是主要的异戊烯醌。全细胞糖为鼠李糖、木糖和甘露糖。主要细胞壁肽聚糖为赖氨酸。该菌株的基因组 DNA G+C 含量为 71.4 mol%。基于 16S rRNA 基因序列的系统发育分析结果表明，菌株 T3246-1 形成了一个与属内其他种密切相关的分支，与 YIM 93306 的相似性为 95.99%。菌株 T3246-1 与 YIM 93306 之间的平均核苷酸同一性值为 76.65%。基于表型和化学分类特征，菌株 T3246-1 被认为代表了属的一个新种，因此提议使用 sp. nov. 来命名这个新种。该模式菌株是 T3246-1（=CGMCC 1.13535=JCM 32923）。
