Zhou Min, Yang Lijie, Chen Yanhong, Sun Tong, Wang Nuan, Chen Xing, Yang Zaibin, Ge Jinshan, Jiang Shuzhen
Department of Animal Sciences and Technology, College of Shandong Agricultural University, Taian, P. R. China.
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Shandong Agricultural University, Taian, P. R. China.
J Anim Physiol Anim Nutr (Berl). 2019 Nov;103(6):1885-1894. doi: 10.1111/jpn.13195. Epub 2019 Sep 4.
The objective of this study was to evaluate the effects of zearalenone (ZEA) and estradiol benzoate (EB) on stress injury and uterine development in post-weaning gilts. Thirty healthy post-weaning female gilts (Duroc × Landrace × Large White) aged 28-32 days were randomly allocated to three treatments as follows: (a) basal diet (Control), (b) basal diet plus 1.0 mg/kg purified ZEA (ZEA) and (c) basal diet plus 0.75 ml (1.5 mg) EB per pig at 3-days intervals by intramuscular injection (EB). The serum estradiol (E ), the final and the increased vulvar area, uterine index, thickness of the myometrium and endometrium, and protein expression of heat shock protein 70 (HSP70) in ZEA group were higher than those in the control group (p < .05), but lower than those in the EB group (p < .05). The serum luteinizing hormone in ZEA group was lower than that of the control group (p < .05), but higher than that in the EB group (p < .05). Higher serum follicle-stimulating hormone and progesterone were observed in the ZEA and control groups than those in the EB group (p < .05). The serum glutathione peroxidase activity in the ZEA group was lower than that in the control and EB groups (p < .001), and the malondialdehyde in the ZEA group was higher than that in the control and EB groups (p < .001). Moreover, the relative mRNA and protein expression of growth hormone receptor (GHR) and relative mRNA expression of HSP70 in the ZEA and EB groups were higher than those in the control group (p < .05). In conclusion, both ZEA (1.0 mg/kg) and EB (1.5 mg at 3 days intervals by intramuscular injection) stimulated vulvar swelling and uterine hypertrophy by disordering serum hormones and up-regulating GHR expression, and induced stress by different mechanisms in this study. Furthermore, the observed up-regulating HSP70 expression challenged by ZEA or EB may be part of the mechanism to resist stress injury.
本研究的目的是评估玉米赤霉烯酮（ZEA）和苯甲酸雌二醇（EB）对断奶后小母猪应激损伤和子宫发育的影响。选取30头28 - 32日龄健康的断奶后雌性小母猪（杜洛克×长白×大白），随机分为以下三组处理：（a）基础日粮（对照组），（b）基础日粮加1.0 mg/kg纯化ZEA（ZEA组），（c）基础日粮加每头猪每隔3天肌肉注射0.75 ml（1.5 mg）EB（EB组）。ZEA组血清雌二醇（E）、外阴最终面积和增加值、子宫指数、子宫肌层和子宫内膜厚度以及热休克蛋白70（HSP70）的蛋白表达均高于对照组（p < 0.05），但低于EB组（p < 0.05）。ZEA组血清促黄体生成素低于对照组（p < 0.05），但高于EB组（p < 0.05）。ZEA组和对照组血清促卵泡生成素和孕酮水平高于EB组（p < 0.05）。ZEA组血清谷胱甘肽过氧化物酶活性低于对照组和EB组（p < 0.001），ZEA组丙二醛含量高于对照组和EB组（p < 0.001）。此外，ZEA组和EB组生长激素受体（GHR）的相对mRNA和蛋白表达以及HSP70的相对mRNA表达均高于对照组（p < 0.05）。总之，在本研究中，ZEA（1.0 mg/kg）和EB（每隔3天肌肉注射1.5 mg）均通过扰乱血清激素和上调GHR表达刺激外阴肿胀和子宫肥大，并通过不同机制诱导应激。此外，ZEA或EB刺激观察到的HSP70表达上调可能是抵抗应激损伤机制的一部分。
