Zeringóta V, de Oliveira Filho J G, Borges L M F
Programa de Pós-Graduação em Ciência Animal, Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, Campus Samambaia, Goiânia, Goiás, Brazil.
Departamento de Biociências e Tecnologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
Med Vet Entomol. 2020 Jun;34(2):236-239. doi: 10.1111/mve.12406. Epub 2019 Sep 4.
The present study aimed to evaluate the behaviour of larvae of Rhipicephalus microplus exposed to different stimuli. A Y-olfactometer was positioned vertically and R. microplus larvae were exposed to environmental air, CO alone, N,N-diethyl-3-methylbenzamide (DEET) alone, and CO combined with the repellents DEET and (E)-2-octenal. Tests were also conducted with the olfactometer positioned horizontally; in this case, however, only CO was tested. In all tests conducted with the Y-olfactometer positioned vertically, CO activated R. microplus larvae even in the presence of DEET and (E)-2-octenal, although activation was lower when these repellents were used. In the absence of CO , larval behaviour against DEET was similar to that of the larvae in the control group. In the tests performed with the olfactometer positioned horizontally, the larvae had no significant response to the presence of CO . The larvae were not attracted to or repelled by any compound tested in either the vertical or horizontal position of the olfactometer. The lack of horizontal displacement, attraction or repellence may have been a result of the ambush behaviour of this tick species. However, when larvae were exposed to stimuli and the olfactometer was positioned vertically, the interference of attractant and repellent stimuli in larval behaviour was assessed.
本研究旨在评估微小扇头蜱幼虫在暴露于不同刺激下的行为。将一个Y型嗅觉仪垂直放置，微小扇头蜱幼虫暴露于环境空气、单独的一氧化碳、单独的N,N - 二乙基 - 3 - 甲基苯甲酰胺（避蚊胺）以及一氧化碳与驱避剂避蚊胺和（E）-2 - 辛烯醛的组合中。还在嗅觉仪水平放置的情况下进行了测试；然而，在这种情况下，仅测试了一氧化碳。在所有使用垂直放置的Y型嗅觉仪进行的测试中，即使存在避蚊胺和（E）-2 - 辛烯醛，一氧化碳仍能激活微小扇头蜱幼虫，尽管使用这些驱避剂时激活程度较低。在没有一氧化碳的情况下，幼虫对避蚊胺的行为与对照组幼虫相似。在嗅觉仪水平放置的测试中，幼虫对一氧化碳的存在没有显著反应。在嗅觉仪垂直或水平放置的情况下，幼虫对所测试的任何化合物均未表现出吸引或排斥。缺乏水平位移、吸引或排斥可能是这种蜱虫伏击行为的结果。然而，当幼虫暴露于刺激且嗅觉仪垂直放置时，评估了引诱剂和驱避剂刺激对幼虫行为的干扰。
