Carroll J F, Solberg V B, Klun J A, Kramer M, Debboun M
United States Department of Agriculture-Agricultural Research Service, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Beltsville, MD 20705, USA.
J Med Entomol. 2004 Mar;41(2):249-54. doi: 10.1603/0022-2585-41.2.249.
The repellents N,N-diethyl-3-methylbenzamide (deet) and racemic 2-methylpiperidinyl-3-cyclohexene-1-carboxamide (AI3-37220) were evaluated using two different laboratory bioassays to determine their relative effectiveness against host-seeking nymphs of the blacklegged tick, Ixodes scapularis Say, and the lone star tick, Amblyomma americanum (L.). In a petri dish bioassay, ticks were released within a ring of repellent on a horizontal filter paper disk. In the second bioassay, ticks were allowed to climb a vertical strip of filter paper whose central portion was treated with a repellent. Deet and AI3-37220 were more effective against I. scapularis than A. americanum nymphs. In the petri dish bioassay, none of the concentrations of deet or AI3-37220 tested confined A. americanum within the treated ring. However, in the vertical bioassay, both species exhibited avoidance of the repellents, and I. scapularis was repelled by much lower concentrations than A. americanum. I. scapularis were repelled by lower concentrations in the vertical bioassay than in the petri dish bioassay. Deet was slightly more effective against I. scapularis than AI3-37220 in both bioassays, but AI3-37220 was significantly more effective than deet against A. americanum in the vertical bioassay.
使用两种不同的实验室生物测定法对驱虫剂N,N - 二乙基 - 3 - 甲基苯甲酰胺（避蚊胺）和外消旋2 - 甲基哌啶基 - 3 - 环己烯 - 1 - 甲酰胺（AI3 - 37220）进行了评估，以确定它们对寻找宿主的黑脚蜱（肩突硬蜱，Say）若虫和美洲钝眼蜱（美洲花蜱，L.）的相对有效性。在培养皿生物测定法中，将蜱虫释放到水平滤纸圆盘上的一圈驱虫剂内。在第二种生物测定法中，让蜱虫攀爬一条垂直的滤纸带，其中心部分用驱虫剂处理。避蚊胺和AI3 - 37220对肩突硬蜱若虫比对美洲花蜱若虫更有效。在培养皿生物测定法中，所测试的避蚊胺或AI3 - 37220的任何浓度都未能将美洲花蜱限制在处理过的圈内。然而，在垂直生物测定法中，两种蜱虫都表现出对驱虫剂的回避，并且肩突硬蜱被比美洲花蜱低得多的浓度驱避。在垂直生物测定法中，肩突硬蜱比在培养皿生物测定法中被更低的浓度驱避。在两种生物测定法中，避蚊胺对肩突硬蜱的效果略优于AI3 - 37220，但在垂直生物测定法中，AI3 - 37220对美洲花蜱的效果明显优于避蚊胺。
