Chen Guoshun, Su Yingyu, Cai Yu, He Lianghong, Yang Gang
College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
Vet Med Sci. 2019 Nov;5(4):526-535. doi: 10.1002/vms3.187. Epub 2019 Sep 4.
The aim of this study was to investigate the effect of dietary mulberry leaves on the transcriptome profiles of finishing pigs. RNA-Seq was used to identify differentially expressed genes (DEGs) in the longissimus dorsi of 56 pigs fed either a traditional diet or diets supplemented with 3%, 6% or 9% mulberry leaf powder, and both gene ontology (GO) function and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analyses were performed. Furthermore, protein-protein interaction (PPI) network and the subnet module analysis were used to identify genes with beneficial potential, and quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate the expression patterns revealed by RNA-Seq.
Pigs fed with the 6% mulberry diet exhibited greater average daily gain, lower water loss and lower shear force than the control group and yielded 531 DEGs, including 271 and 260 upregulated and downregulated genes, respectively. Function analysis revealed that the DEGs were significantly enriched in functions related to muscle growth and development. Furthermore, several genes (i.e. ACOT4, ECHS1, HACD1, NPR1, ADCY2, MGLL and IRS1) were enriched in a KEGG pathway that was associated with fatty acid metabolism, and in the PPI subnet module, four of eight node genes, namely TNNC1, MYL3, TCAP and TNNT1, were associated with muscle formation and development. The upregulation of these genes, including TNNC1, TNNT1 and MYL3, was confirmed by qRT-PCR.
Dietary mulberry leaves (6%) may improve the muscle quality of pigs by modulating the expression of several key genes, such as TNNC1, MYL3 and TNNT1.
本研究旨在探讨日粮中添加桑叶对育肥猪转录组图谱的影响。采用RNA测序技术鉴定56头分别饲喂传统日粮或添加3%、6%或9%桑叶粉日粮的猪背最长肌中的差异表达基因（DEG），并进行基因本体（GO）功能和京都基因与基因组百科全书（KEGG）通路富集分析。此外，利用蛋白质-蛋白质相互作用（PPI）网络和子网模块分析来鉴定具有潜在益处的基因，并采用定量实时聚合酶链反应（qRT-PCR）验证RNA测序所揭示的表达模式。
与对照组相比，饲喂6%桑叶日粮的猪平均日增重更高、失水率更低、剪切力更低，共产生531个差异表达基因，其中上调基因271个，下调基因260个。功能分析表明，差异表达基因在与肌肉生长发育相关的功能中显著富集。此外，几个基因（即ACOT4、ECHS1、HACD1、NPR1、ADCY2、MGLL和IRS1）在与脂肪酸代谢相关的KEGG通路中富集，在PPI子网模块中，八个节点基因中的四个，即TNNC1、MYL3、TCAP和TNNT1，与肌肉形成和发育相关。qRT-PCR证实了这些基因（包括TNNC1、TNNT1和MYL3）的上调。
日粮中添加6%桑叶可能通过调节TNNC1、MYL3和TNNT1等几个关键基因的表达来改善猪的肌肉品质。
